| 现场裂解猪全血提取DNA |
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| 引用本文： | 李雪梅,龚炎长,赵书红,李奎,彭中镇,师守坤.现场裂解猪全血提取DNA[J].河北农业大学学报,1997(4). |
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| 作者姓名： | 李雪梅 龚炎长 赵书红 李奎 彭中镇 师守坤 |
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| 作者单位： | 华中农业大学动物分子生物学与育种实验室!武汉，430070，华中农业大学动物分子生物学与育种实验室!武汉，430070，华中农业大学动物分子生物学与育种实验室!武汉，430070，华中农业大学动物分子生物学与育种实验室!武汉，430070，华中农业大学动物分子生物学与育种实验室!武汉，430070 |
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| 摘 要： | 为了解决常规现场裂解猪全血时出现的血液凝结问题，提高了裂解液中抗凝剂EDTA的浓度，并对血液消化的蛋白酶K的用量作了梯度处理。结果经方差分析表明：不同裂解液间DNA产量有极显著差异（P＜0．001）。用我们改进的抗凝剂配方，DNA产量高于常规裂解波，且所提DNA质量没有下降；不同蛋白酶K用量间无显著差异（P＞0．05）。
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| 关 键 词： | 猪 DNA 裂解 |
DNA Extraction from Porcine Blood Lysed in Pig Farm |
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| Li Xuemei, Gong Yanzhan, Zhao Shuhong,Li Kui, Peng Zhongzhen.DNA Extraction from Porcine Blood Lysed in Pig Farm[J].Journal of Agricultural University of Hebei,1997(4). |
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| Authors: | Li Xuemei Gong Yanzhan Zhao Shuhong Li Kui Peng Zhongzhen |
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| Abstract: | In order to solve the problem of porcine blood condensation during the course of collection and transport, different concentration of EDTA, an antithrombotics, and gradient concentration of proteinase K were used in the experiment. Varience analysis showed that DNA output had significant difference (P<0. 001) between diferent lysates. Using our lysate, DNA output was higher than that of using normal method but DNA quality was not affected. There is no significant difference between different Proteinase K concentration (P>0. 05). |
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| Keywords: | Pig NDA Lysis |
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