| 二色胡枝子组培再生体系优化的研究 |
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| 引用本文： | 黄天伟,李伟,李明,陈晓阳.二色胡枝子组培再生体系优化的研究[J].河北林果研究,2009,24(2):117-120. |
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| 作者姓名： | 黄天伟 李伟 李明 陈晓阳 |
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| 作者单位： | 1. 北京林业大学,林木花卉遗传育种教育部重点实验室,北京,100083
2. 北京林业大学,林木花卉遗传育种教育部重点实验室,北京,100083;华南农业大学林学院,广东,广州,510642 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 为了实现二色胡枝子高效再生体系的优化，以二色胡枝子为材料，研究了6-BA、2，4-D、NAA等因素对其子叶节再生、节段扩繁和试管苗生根的影响。试验结果表明：子叶节再生最佳培养基为MS＋6一BA0．1mg／L；最佳增殖培养基为MS＋6-BA0．1mg／L＋NAA 0．01mg／L；IBA，NAA对二色胡枝子试管苗的生根作用有影响，最佳生根培养基为1／2MS＋IBA0．5mg／或者1／2MS＋NAA0．1mg／L。
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| 关 键 词： | 二色胡枝子 再生体系优化 增殖 生根 |
Studies on optimizing Lespedeza bicolor regeneration system |
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| HUANG Tian-wei,LI Wei,LI Ming,CHEN Xiao-yang.Studies on optimizing Lespedeza bicolor regeneration system[J].Hebei Journal of Forestry and Orchard Research,2009,24(2):117-120. |
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| Authors: | HUANG Tian-wei LI Wei LI Ming CHEN Xiao-yang |
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| Institution: | 1 Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants;Ministry of Education;Beijing Forestry University;Beijing 100083;China;2 College of Forestry;South China Agricultural University;Guangzhou 510642;China |
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| Abstract: | In order to optimize the effective regeneration system of Lespedeza bicolor, the effects of 6-BA,2,4-D,NAA on cotyledonary node regeneration,proliferation and rooting of Lespedeza bicolor were studied.The results showed that the optimum regeneration medium was MS+6-BA 0.1mg/L.MS+6-BA 0.1 mg/L +NAA 0.01 mg/L was an optimum proliferation medium.IBA and NAA played an important role in rooting and the optimum rooting medium was 1/2MS +IBA 0.5 mg/L or 1/2MS +NAA 0.1mg/L. |
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| Keywords: | Lespedeza bicolor optimization of regeneration system proliferation rooting |
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