| 杨树PtROP家族基因的表达分析与功能预测 |
| |
| 引用本文： | 李煜,张进,王丽娟,卢孟柱.杨树PtROP家族基因的表达分析与功能预测[J].林业科学研究,2017,30(1):1-9. |
| |
| 作者姓名： | 李煜 张进 王丽娟 卢孟柱 |
| |
| 作者单位： | 林木遗传育种国家重点实验室, 中国林业科学研究院林业研究所, 北京 100091;国家林业局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091;林木遗传育种国家重点实验室, 中国林业科学研究院林业研究所, 北京 100091;国家林业局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091;林木遗传育种国家重点实验室, 中国林业科学研究院林业研究所, 北京 100091;国家林业局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091;林木遗传育种国家重点实验室, 中国林业科学研究院林业研究所, 北京 100091;国家林业局林木培育重点实验室, 中国林业科学研究院林业研究所, 北京 100091 |
| |
| 基金项目： | 国家自然科学基金面上项目（31270699）。 |
| |
| 摘 要： | 目的]为探究ROP基因在林木中的功能。方法]本研究以杨树为模式,在全基因组水平上对ROP基因的家族成员、基因结构、保守结构域、氨基酸序列相似性及表达模式进行了分析。结果]显示,杨树PtROP基因家族包含13个成员,不同成员间在进化上相对保守,均存在与GTP结合与水解相关的结构域。这些基因在不同组织、不同胁迫条件下的表达具有明显差异,说明它们参与不同的生物学过程。通过构建功能基因网络(functional gene network for poplar)发现PtROP基因主要参与了信号转导过程。结论]杨树PtROP基因家族包含13个成员参与不同的生物学过程,可能主要参与了信号转导过程。
|
| 关 键 词： | 杨树 ROP 基因家族 基因表达模式 |
| 收稿时间： | 2016/2/1 0:00:00 |
Expression and Functional Analysis of ROP Gene Family in Populus |
| |
| LI Yu,ZHANG Jin,WANG Li-juan and LU Meng-zhu.Expression and Functional Analysis of ROP Gene Family in Populus[J].Forest Research,2017,30(1):1-9. |
| |
| Authors: | LI Yu ZHANG Jin WANG Li-juan and LU Meng-zhu |
| |
| Institution: | State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China |
| |
| Abstract: | Objective] lore the putative function of ROPs in forest trees.Method] A genome-wide analysis of ROPs was performed, including the phylogeny, gene structure, conserved motifs, ROP amino sequences similarity between Populus and Arabidopsis ROP proteins and the expression patterns using Populus as a model.Result] The results showed that there were 13 members of PtROP genes in Populus and the PtROP genes were conserved during evolution of species, all of them containing GTP binding and hydrolysis-related domains. Furthermore, the expression profiles revealed that Populus PtROP had distinct expression pattern across different tissues and different stress conditions, suggesting that PtROP genes were involved in different biological processes. In addition, the analysis on PtROP gene functional network was conducted. It is predicted that Populus PtROP genes were mainly involved in signal transduction.Conclude] In this study, systematically bioinformatics analysis on PtROP were conducted which laid the foundation for the further exploration of poplar PtROP functions. |
| |
| Keywords: | Populus ROP gene family gene expression pattern |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《林业科学研究》浏览原始摘要信息 |
| 点击此处可从《林业科学研究》下载免费的PDF全文 |
|
