| 甘蓝型油菜与诸葛菜属间杂交F12群体的品质及遗传分析 |
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| 引用本文： | 徐传远,李再云.甘蓝型油菜与诸葛菜属间杂交F12群体的品质及遗传分析[J].中国油料作物学报,2011,33(1):20-24. |
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| 作者姓名： | 徐传远 李再云 |
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| 作者单位： | 1. 河北理工大学化工与生物技术学院，河北 唐山 063009；
2. 华中农业大学作物遗传改良国家重点实验室，湖北 武汉 430070）
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| 基金项目： | 国家重点实验室专项经费; 唐山市科技局项目(09120202c) |
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| 摘 要： | 以甘蓝型油菜（AACC，2n=38）品种Oro与诸葛菜（OO，2n=24）属间杂交F4世代中出现的五倍体单株（AACCO，2n=50）为起始材料连续自交选育、鉴定，直至获得F12群体。各F12株系间表现一定的形态差异, 但株系内植株形态特征一致。细胞学观察表明85.3%的F12单株具有与甘蓝型油菜相同的染色体数目，花粉母细胞(PMCs) 减数分裂中正常配对和分离；其余植株的染色体数目分别为37、39和40；红紫色植株在减数分裂中有落后染色体，四分体时期会出现微核。基因组原位杂交(Genomic in situ hybridization，GISH)分析未检测出整条的诸葛菜染色体；但AFLP分析表明存在渗入的诸葛菜DNA片段。F12植株的脂肪酸组成及硫苷含量表现较大的变异, 有的植株油酸含量高于Oro而硫苷降低。
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| 关 键 词： | 甘蓝型油菜 诸葛菜 基因渗入 属间杂种 |
Seed quality and genetic analysis of F12 progenies from intergeneric hybrids between Brassica napus and Orychophragmus violaceus |
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| XU Chuan-yuan,LI Zai-yun.Seed quality and genetic analysis of F12 progenies from intergeneric hybrids between Brassica napus and Orychophragmus violaceus[J].Chinese Journal of Oil Crop Sciences,2011,33(1):20-24. |
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| Authors: | XU Chuan-yuan LI Zai-yun |
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| Institution: | XU Chuan-yuan1,LI Zai-yun2*(1.College of Chemical Engineering and Biological Technology,Hebei Polytechnic University,Tangshan 063009,China,2.National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China) |
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| Abstract: | In F4 progenies from intergeneric hybrids between Brassica napus(AACC,2n=38) cv.Oro and Orychophragmus violaceus(OO,2n=24),a pentaploid plant(AACCO,2n=50) was found and selfed consecutively to F12 generation.Every F12 line showed distinct phenotype,but plants from the same line had uniform morphology.Cytological investigations revealed that 85.3% of the F12 plants had the same chromosome numbers(2n=38) as B.napus,which were paired as 19 bivalents and segregated equally as 19:19 during the meiosis of pollen ... |
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| Keywords: | Brassica napus Orychophragmus violaceus Genetic introgression Intergeneric hybrid |
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