| 1株白魔芋软腐病病原菌鉴定及其室内药剂筛选 |
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| 引用本文： | 罗林丽,赵兴丽,周罗娜,贺圣凌,刘思睿,张欣,罗可,周玉锋.1株白魔芋软腐病病原菌鉴定及其室内药剂筛选[J].南方农业学报,2022,53(11):3137-3146. |
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| 作者姓名： | 罗林丽 赵兴丽 周罗娜 贺圣凌 刘思睿 张欣 罗可 周玉锋 |
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| 作者单位： | 1. 贵州省农业科学院生物技术研究所, 贵州贵阳 550006;2. 贵州省农业生物技术重点实验室, 贵州贵阳 550006;3. 贵州省农业科学院茶叶研究所, 贵州贵阳 550006 |
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| 基金项目： | 贵州省科技计划项目(黔科合服企[2019]4009,黔科合服企[2021]11号,黔科合支撑[2021]一般208) |
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| 摘 要： | 【目的】明确贵州省兴义市安龙县白魔芋软腐病的病原菌种类,并探究杀菌剂对病原菌株的室内抑制活性,以期为白魔芋软腐病田间防治提供理论依据。【方法】利用平板划线分离法对采集自贵州省安龙县白魔芋种植基地具有软腐病典型症状的白魔芋茎部组织进行病原菌分离和纯化,利用柯赫氏法则对分离所得的菌株进行致病性检测,结合形态特征、16S rDNA序列分析及系统发育进化树分析对病原菌株进行分类鉴定。选取13种常见杀菌剂,采用抑菌圈法测定不同杀菌剂对病原菌的室内毒力。【结果】确定引起贵州省安龙县白魔芋软腐病的病原为环状果胶杆菌(Pectobacterium aroidearum),菌株编号XYmy-A1。室内毒力测定结果显示,供试杀菌剂中有7种药剂对菌株XYmyA1生长有一定的抑制作用,80%乙蒜素乳油、5%氨基寡糖素·20%乙蒜素微乳剂、25%溴菌腈微乳剂、3%中生菌素可溶液剂和0.3%四霉素水剂在供试浓度下对病原菌生长均具有抑制作用,而1.5%噻霉酮水乳剂和1.8%辛菌胺醋酸盐水剂只有在高浓度下对病原菌生长具有抑制作用;其中,0.3%四霉素对菌株XYmy-A1的抑制效果最强,其对病原菌的抑制中浓度(EC50)为0.1980 mg/L,其次是25%溴菌腈微乳剂,其EC50为9.1359 mg/L。【结论】引起贵州省安龙县白魔芋软腐病的病原菌为环状果胶杆菌,可首选0.3%四霉素水剂和25%溴菌腈微乳剂进一步开展田间防效试验,1.5%噻霉酮水乳剂、3%中生菌素可溶液剂、80%乙蒜素乳油和5%氨基寡糖素·20%乙蒜素微乳剂可作为备选药剂。
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| 关 键 词： | 白魔芋 软腐病 病原 药剂筛选 |
| 收稿时间： | 2022-02-22 |
Identification of a pathogen causing soft rot on Amorphophallus albus and laboratory screening for bactericide |
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| LUO Lin-li,ZHAO Xing-li,ZHOU Luo-na,HE Sheng-ling,LIU Si-rui,ZHANG Xin,LUO Ke,ZHOU Yu-feng.Identification of a pathogen causing soft rot on Amorphophallus albus and laboratory screening for bactericide[J].Journal of Southern Agriculture,2022,53(11):3137-3146. |
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| Authors: | LUO Lin-li ZHAO Xing-li ZHOU Luo-na HE Sheng-ling LIU Si-rui ZHANG Xin LUO Ke ZHOU Yu-feng |
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| Institution: | 1. Institute of Biotechnology, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550006, China;2. Guizhou Key Laboratory of Agricultural Biotechnology, Guiyang, Guizhou 550006, China;3. Tea Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550006, China |
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| Abstract: | 【Objective】In order to provide reference for field bactericide control,this paper identified the pathogen species of Amorphophallus albus soft rot in Anlong County,Xingyi City,Guizhou Province,and explored the indoor inhibitory activity of several bactericides against the pathogenic strain.【Method】The pathogen were isolated and purified from diseased stalk of A. albus by streak plate method sampled from planting base in Anlong County,Guizhou Province,the pathogenicity of the isolates were detected by Koch's postulate,the pathogen was identified by morphological characteristics,16S rDNA sequence analysis and phylogenetic tree analysis. The indoor toxicity of thirteen common agents to pathogen were measured by bacteriostatic circle method.【Result】It was confirmed that Pectobacterium aroidearum was the causal agent of the A. albus soft rot,which was found in Anlong County,Guizhou Province,and the strain was named as XYmy-A1. The results of indoor toxicity measurement indicated that there were seven bactericides of experimental pesticides had inhibitory effect on the growth of XYmy-A1,80% ethylicin EC,5% oligosaccharins ·20% ethylicin ME,25% brothermothingonil ME,3% zhongshengmycin SL,0.3% tetramycin AS had inhibitory effects on the growth of pathogen at the tested concentration,while 1.5% benziothiazolinone EW and 1.8% xinjunan acetate AS had inhibitory effects on strain only at high concentration. Among them,0.3% tetramycin had the strongest inhibitory effect to XYmyA1,the median inhibition concentration(EC50) was 0.1980 mg/L,and followed by 25% bromothalonil,whose EC50 was 9.1359 mg/L.【Conclusion】The pathogen is P. aroidearum which causing soft rot on A. albus in Anlong County,Guizhou Province. 0.3% ehydroxide AS and 25% bromothalonil ME can be selected for the first choice for field trials,1.5% benziothiazolinone EW,3% zhongshengmycin SL,80% ethylicin and 5% oligosaccharins·20% ethylicin can be used as alternative agents. |
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