| 一株具有烟草甲毒杀作用的苏云金芽孢杆菌对烟叶的影响 |
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| 引用本文： | 胡逸超,苏赞,雷薇,陈义昌,邹克兴,陈琦,邹承武.一株具有烟草甲毒杀作用的苏云金芽孢杆菌对烟叶的影响[J].南方农业学报,2022,53(6):1568-1576. |
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| 作者姓名： | 胡逸超 苏赞 雷薇 陈义昌 邹克兴 陈琦 邹承武 |
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| 作者单位： | 1. 广西中烟工业有限责任公司技术中心, 广西南宁 530001;2. 南宁国拓生物科技有限公司, 广西南宁 530001;3. 广西大学农学院/植物科学国家级实验教学示范中心, 广西南宁 530004 |
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| 基金项目： | 广西中烟工业有限责任公司科技项目(GXZYCX 2019H002,GXZYCX2017B005)广西工业创新发展项目(科技20190029)广西创新驱动发展专项(桂科AA17204054) |
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| 摘 要： | 【目的】明确一株具有烟草甲毒杀作用的苏云金芽孢杆菌（Bacillus thuringiensis，Bt）在醇化过程中对烟叶的影响，为烟叶仓库中应用Bt菌剂防控烟草甲的可行性和安全性评价提供参考依据。【方法】按照0.1 g Bt菌剂处理1 kg烟叶的比例作处理，以未喷施Bt菌剂的烟叶作为对照，在仓库中贮藏12个月后，每隔...
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| 关 键 词： | 苏云金芽孢杆菌 烟叶 醇化 化学成分 感官质量 细菌群落结构多样性 |
| 收稿时间： | 2021-11-16 |
Effects of Bacillus thuringiensis with toxics for Lasioderma serricorne on tobacco leaves |
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| HU Yi-chao,SU Zan,LEI Wei,CHEN Yi-chang,ZOU Ke-xing,CHEN Qi,ZOU Cheng-wu.Effects of Bacillus thuringiensis with toxics for Lasioderma serricorne on tobacco leaves[J].Journal of Southern Agriculture,2022,53(6):1568-1576. |
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| Authors: | HU Yi-chao SU Zan LEI Wei CHEN Yi-chang ZOU Ke-xing CHEN Qi ZOU Cheng-wu |
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| Institution: | 1. Technical Center, China Tobacco Guangxi Industrial Co., Ltd., Nanning, Guangxi 530001, China;2. Nanning Goldtech Bio-science Co., Ltd., Nanning, Guangxi 530001, China;3. College of Agriculture, Guangxi University/National Demonstration Center for Experimental Plant Science Education, Nanning, Guangxi 530004, China |
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| Abstract: | 【Objective】 To investigate effects of a strain of Bacillus thuringiensis（Bt）with toxics for Lasioderma serricorne on tobacco leaves during alcoholization，so as to provide reference for safety and feasibility accession by applying Bt bacterial agent to prevent and control L.serricorne in tobacco warehouses.【Method】 Tobacco leaves was treated with microbial agent at a ratio of 0.1 g Bt ratio to 1 kg tobacco and the untreated tobacco was taken as control.After 12 months of storage in warehouse，the tobacco leaves were collected every 2 months.Seven chemical indexes，including total sugar，reducing sugar，chlorine，total alkaloid，potassium，total nitrogen and starch，were detected.Ten items of sensory evaluation were also measured and accessed，namely aroma quality，aroma volume，offensive odor，irritancy，permeability，softness，sweetness，aftertaste，concentration and intensity.After alcoholization for 24 months，the bacteria diversity of tobacco leaves was analyzed using 16S rDNA amplicon sequencing to detect difference of changes in bacterial community structure diversity between the Bt treatment and control groups.【Result】 The 7 chemical indexes did not show significant change in the Bt bacterial agent treatment（P>0.05）.Five sensory items significantly changed（P<0.05，the same below），in which aroma volume and offensive odor were better in the treatment group，while the sensory evaluation scores of aroma quality，permeability and sweetness were lower.The bacterial species between these two groups were significantly different.Bacterial species of treatment group and control group varied obviously: in the treatment group，among class，order，family，and genus levels，relative abundance were higher in Bacilli（95.9%），Bacillales（95.9%），Bacillaceae （96.0%），and Bacillus（95.9%），respectively；in control group，relative abundance were higher in Gammaproteobacteria （47.6%），Enterobacteriales（31.3%），Enterobacteriaceae（33.7%），Escherichia-Shigella（22.9%）.Seem from Alpha diversity indexes，Observed species index and Chao1 index had significant difference，indicating that the bacterial community diversity of the treatment group was lower than that the control group.Beta diversity analysis proved that significant difference of bacterial community structure existed between the two groups.【Conclusion】 In the Bt treatment group，the bacterial community diversity in tobacco during alcoholization had significant difference，but such treatment has little change in chemical components and sensory quality of tobacco，with no quality decline found，indicating low economic risk of the applying Bt in L.serricorne control. |
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