| 不同苗种来源香港牡蛎的生长差异及基因表达相关分析 |
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| 引用本文： | 严雪瑜,蒋美君,钟方杰,黄琮荔,张斌,彭金霞,朱鹏,李选积,覃波忠,何苹萍.不同苗种来源香港牡蛎的生长差异及基因表达相关分析[J].南方农业学报,2022,53(12):3568-3575. |
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| 作者姓名： | 严雪瑜 蒋美君 钟方杰 黄琮荔 张斌 彭金霞 朱鹏 李选积 覃波忠 何苹萍 |
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| 作者单位： | 1 北部湾大学海洋学院/广西北部湾海洋生物多样性养护重点实验室, 广西钦州 535011;2 钦州市水产技术推广站, 广西钦州 535000;3 广西水产科学研究院/广西水产遗传育种与健康养殖重点实验室, 广西南宁 530021 |
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| 基金项目： | 广西重点研发计划项目（桂科AB21196030）；广西自然科学基金青年基金项目（2019GXNSFBA245081）；广西水产遗传育种与健康养殖重点实验室开放项目（GXKEYLA2020-01）；钦州市科学研究与技术开发项目（20221214） |
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| 摘 要： | 【目的】明确不同苗种来源香港牡蛎的生长差异及生长相关基因表达模式，为筛选出生长性状候选基因打下理论基础，也为繁育优质香港牡蛎新品种提供参考依据。【方法】通过比较人工繁育（人工组）和自然海域半人工采苗（天然组） 2种模式获得香港牡蛎的生长差异，利用实时荧光定量PCR检测不同苗种来源香港牡蛎外套膜和闭壳肌中IGF1、AMPKα和FoxO1基因的表达情况，并利用统计学方法及相关分析探究生长指标（壳高、壳长、壳宽、鲜重和软体重）与生长相关基因表达变化间的相关性。【结果】人工组和天然组香港牡蛎在试验期间（4月龄~12月龄）的壳高、壳长、壳宽、鲜重和软体重等生长指标整体上呈增长趋势；人工组香港牡蛎的生长指标均显著高于天然组香港牡蛎（P<0.05，下同），但天然组香港牡蛎生长指标的整体增幅高于人工组香港牡蛎。天然组香港牡蛎外套膜和闭壳肌中IGF1、AMPKα和FoxO1基因在不同发育阶段的相对表达量整体上高于人工组香港牡蛎，但差异均不显著（P>0.05，下同）。相关分析结果表明，人工组和天然组香港牡蛎生长相关基因表达与生长性状间具有相关性，其中外套膜和闭壳肌的IGF1基因表达总量与壳高呈负相关，与壳宽呈正相关； AMPKα基因表达总量与壳高、鲜重和软体重等生长指标呈正相关，与壳宽呈负相关； FoxO1基因表达总量与壳宽呈负相关。【结论】无论是人工繁育还是自然海域半人工采苗获得的香港牡蛎，在生长性状及相关基因表达方面均存在一定差异。其中，IGF1、AMPKα和FoxO1基因参与调控香港牡蛎的生长发育，且对不同生长指标有不同的指示作用，因此开展人工繁育时可通过调控这些基因的表达以改变香港牡蛎生长指标的增幅。
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| 关 键 词： | 香港牡蛎 人工繁育 自然海区半人工采苗 生长差异 基因表达 |
| 收稿时间： | 2022-05-14 |
Correlation analysis between growth differences and gene expression in different breeding sources of Crassostrea hongkongensis |
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| YAN Xue-yu,JIANG Mei-jun,ZHONG Fang-jie,HUANG Cong-li,ZHANG Bin,PENG Jin-xia,ZHU Peng,LI Xuan-ji,QIN Bo-zhong,HE Ping-ping.Correlation analysis between growth differences and gene expression in different breeding sources of Crassostrea hongkongensis[J].Journal of Southern Agriculture,2022,53(12):3568-3575. |
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| Authors: | YAN Xue-yu JIANG Mei-jun ZHONG Fang-jie HUANG Cong-li ZHANG Bin PENG Jin-xia ZHU Peng LI Xuan-ji QIN Bo-zhong HE Ping-ping |
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| Institution: | 1 College of Marine Sciences, Beibu Gulf University/Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou, Guangxi 535011, China;2 Qinzhou Aquatic Product Technology Promotion Department, Qinzhou, Guangxi 535000, China;3 Guangxi Academy of Fisheries Sciences/Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, Guangxi 530021, China |
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| Abstract: | 【Objective】 Growth differences during the different seedling sources of Crassostrea hongkongensis and their correlations with the expression patterns of growth genes were investigated to provide a theoretical basis for screening candidate genes for growth traits and provide reference for breed selection of C. hongkongensis.【Method】 Growth differences were observed by comparing C. hongkongensis derived from artificial spat-collection(artificial group) and semiartificial collection of seedling in natural ocean systems(natural group). The expression of IGF1, AMPKα and FoxO1 genes in the mantles and adductor muscles of C. hongkongensis was deduced by real-time fluorescence quantitative PCR. Correlation was analyzed between growth traits(shell height, shell length, shell width, whole weight and soft tissue weight) and growth-related gene expression by statistical methods and correlation analysis.【Result】 Growth traits including shell height, shell length, shell width, whole weight and soft tissue weight of C. hongkongensis obtained from artificial group and natural group showed an overall increasing trend during the experiment(4-month-old to 12-month-old). The growth traits of artificial group were significantly higher than natural group(P<0.05, the same below). However, the growth rate of natural group was higher than that of artificial group. The gene expression of IGF1, AMPKα and FoxO1 in the mantle and adductor muscle in natural group were higher than those in artificial group at different developmental stages, but the differences were not significant(P>0.05, the same below). Correlation analysis results showed that there was correlation between growth-related genes and growth traits of the artificial group and natural group. The total expression of IGF1 in the mantle and adductor muscle was negatively correlated with shell height and positively correlated with shell width. The total expression of AMPKα was positively correlated with growth traits including shell height, whole weight and soft tissue weight, and negatively correlated with shell width. The total expression of FoxO1 was negatively correlated with shell width.【Conclusion】 There are some differences in growth traits and growth-related genes expression between C. hongkongensis obtained by artificial spat-collection and semi artificial seedling collection in natural sea areas. IGF1, AMPKα and FoxO1 genes play a certain regulatory role in the growth of C. hongkongensis, and have different indicating effects on different growth indexes. It is speculated that artificial breeding can alter the increase rate of growth indicators of C. hongkongensis by regulating the amounts of IGF1, AMPKα and FoxO1 genes expression. |
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