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一株番茄青枯病拮抗细菌JK19的鉴定及其抑菌活性检测
引用本文:黄伟,宋博,张丽娟,王博,王玮.一株番茄青枯病拮抗细菌JK19的鉴定及其抑菌活性检测[J].新疆农业科学,2021,58(7):1355-1364.
作者姓名:黄伟  宋博  张丽娟  王博  王玮
作者单位:1.新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091;2.新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物防治综合治理重点实验室/农业部库尔勒作物有害生物科学观测试验站,乌鲁木齐 830091
基金项目:自治区重点实验室开放课题“基于OSMAC策略的耐辐射链霉菌A144农用天然产物挖掘与研究”(2020D04032);新疆农业科学院青年基金“青枯病拮抗菌的筛选、鉴定及其活性物质的初步分析”(xjnkq-2018010);国家生物农药工程技术研究中心及农业部农业微生物资源利用华中区域科学观测试验站联合开放基金”(JF-NBCOES-1802);新疆农业科学院青年科技骨干创新能力培养项目“一株高效杀线虫芽孢杆菌活性物质提取及作用机制研究”(xjnkq-2019019)
摘    要:【目的】研究拮抗细菌JK19的分类学地位,检测其抑菌活性,分析其可能的活性成分。为脂肽提取和生防制剂的研制提供了理论依据。【方法】采用形态学、生理生化特征及16S rDNA序列分析方法,鉴定菌株;采用平板对峙法检测菌株对8种植物病原菌的拮抗作用;采用PCR扩增方法检测基因组中的脂肽类抗生素合成及促生作用相关的基因。【结果】鉴定JK19为芽孢杆菌,JK19与贝莱斯芽孢杆菌、暹罗芽孢杆菌、解淀粉芽孢杆菌同源关系比较近。菌株JK19对多种植物病原菌具有明显的抑制作用,抑制效果分别为香梨黑斑病 66.67%、核桃腐烂病100%、马铃薯早疫病73.33%、西瓜枯萎病 60.71%、水稻恶苗病67.86%、棉花枯萎病 76.92%、西瓜蔓枯病100%。PCR反应检测JK19基因组中有参与脂肽类抗生素合成相关的基因 ituA、ituD、bamC、fenB、fenD、srfAB;参与生长素IAA合成的基因ysnE;促生作用相关的基因yndJ【结论】拮抗细菌JK19为芽孢杆菌属细菌,对番茄青枯病病原菌等多种植物病原菌均有明显的抑制作用,其基因组中含有脂肽类和促生作用相关基因。

关 键 词:芽孢杆菌  抑菌谱  脂肽类抗生素  
收稿时间:2020-05-13

Identification of Bacterial Wilt Antagonistic Bacteria JK19 and Detection of Its Antibacterial Activity
HUANG Wei,SONG Bo,ZHANG Lijuan,WANG Bo,WANG Wei.Identification of Bacterial Wilt Antagonistic Bacteria JK19 and Detection of Its Antibacterial Activity[J].Xinjiang Agricultural Sciences,2021,58(7):1355-1364.
Authors:HUANG Wei  SONG Bo  ZHANG Lijuan  WANG Bo  WANG Wei
Institution:1. Xinjiang Laboratory of Special Environmental Microbiology /Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China;2. Key Laboratory of Integrated Crop Pest Management in Northwestern Oasis of China,MOARA/ Scientific Observing and Experimental Station of Crop Pests of MOARA in Korla / Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China
Abstract:【Objective】 To clarify the taxonomic status of antagonistic bacterium JK19, detect its antibacterial activity, and analyze its possible active components. The results provide theoretical basis for the extraction of lipopeptide and the development of biological control agents .【Methods】 The strain was identified by morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis. The antagonistic effect of the strain against 8 plant pathogens was detected by plate confrontation method. PCR amplification was used to detect the genes related to the synthesis and probiotics of lipopeptide antibiotics in the genome.【Results】 JK19 was identified as bacillus by morphological, physiological and biochemical characteristics, and 16S rDNA sequences of JK19 were compared to construct a developmental tree. It was found that JK19 had close homologous relations with Bacillus velezensis, Bacillussiamensis and Bacillus amylobacillus. Strain JK19 had significant inhibition effect on various plant pathogens, and the inhibition effect was 66.67% for black spot of fragrant pear, 100% for walnut rot, 73.33% for potato early blight, 60.71% for watermelon blight, 67.86% for rice blight, 76.92% for cotton blight and 100% for watermelon vine blight, respectively. PCR reaction detected the genes involved in the synthesis of lipopeptide antibiotics in JK19 genome: ituA, ituD, bamC, fenB, fenD, srfAB, YsnE, a gene involved in the synthesis of auxin IAA and geneyndJassociated with probiotics.【Conclusion】 Antagonistic bacterium JK19 is abacillussp., which has obvious inhibitory effect on various plant pathogenic bacteria such as tomato bacterial wilt, and its genome contains lipopeptide and genes related to probiotics.
Keywords:Bacillus sp    inhibition spectrum  lipopeptide antibiotics  
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