| 基于飞行时间质谱法的单核细胞增生李斯特氏菌定性质控样品研制 |
| |
| 引用本文： | 张雅伦,杨帆,张涛,张瑞,李献,周巍,张岩.基于飞行时间质谱法的单核细胞增生李斯特氏菌定性质控样品研制[J].乳业科学与技术,2022,45(6):27-32. |
| |
| 作者姓名： | 张雅伦 杨帆 张涛 张瑞 李献 周巍 张岩 |
| |
| 作者单位： | （河北省食品检验研究院, 河北省食品安全重点实验室, 国家市场监督管理重点实验室（特殊食品监管技术）, 特殊食品安全与健康河北省工程研究中心, 河北 石家庄 050071） |
| |
| 基金项目： | 河北省市场监督管理局科研计划项目（2022ZD12） |
| |
| 摘 要： | 通过真空冷冻干燥技术成功制备出单核细胞增生李斯特氏菌定性质控样品，并系统分析质控样品的均匀性和稳定性。通过优化冻干基质条件，得到制备质控样品的最佳条件。通过培养计数和基质辅助激光解吸电离飞行时间质谱技术，验证定性质控样品的均匀性和稳定性。结果表明：制备的单增李斯特菌定性质控样品为白色、质地均匀小球，均匀性验证实验质控样品培养计数结果F=0.567，小于临界值，表明均匀性一致；运输稳定性实验验证了质控样品在37、25?℃环境下含量几乎没有下降，计数稳定；贮藏稳定性实验验证了质控样品在－20?℃贮藏28?d后的复苏率为101.5%，在4?℃条件下贮藏28?d后的复苏率为99.6%，说明该样品均匀性和稳定性良好，可以作为阳性质控样品用于单增李斯特菌检测和质量控制。
|
| 关 键 词： | 基质辅助激光解吸电离飞行时间质谱技术 单核细胞增生李斯特氏菌 定性质控样品 均匀性 稳定性 |
Development of Quality Control Samples for Qualitative Analysis of Listeria monocytogenes by Time-of-Flight Mass Spectrometry |
| |
| ZHANG Yalun,YANG Fan,ZHANG Tao,ZHANG Rui,LI Xian,ZHOU Wei,ZHANG Yan.Development of Quality Control Samples for Qualitative Analysis of Listeria monocytogenes by Time-of-Flight Mass Spectrometry[J].JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY,2022,45(6):27-32. |
| |
| Authors: | ZHANG Yalun YANG Fan ZHANG Tao ZHANG Rui LI Xian ZHOU Wei ZHANG Yan |
| |
| Institution: | (Hebei Engineering Research Center for Special Food Safety and Health, Key Laboratory of Special Food Supervision Technology for State Market Regulation, Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050071, China) |
| |
| Abstract: | A quality control sample for the qualitative analysis of Listeria monocytogenes was developed by vacuum freezing drying technology, and the homogeneity and stability of the quality control sample were systematically analyzed. By optimizing the type of lyophilized matrix, the optimal conditions for the development of quality control samples were obtained. The homogeneity and stability of the developed quality control sample were verified by counting the number of cells as well as using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The quality control sample was white in color, spherical in shape and uniform in size. The results of cell counting for uniformity validation showed F = 0.567, which was less than the critical value, indicating good uniformity. In the transportation stability test, the quality control sample remained stable at 37 and 25 ℃. In the storage stability test, the resurrection rate of the quality control sample was 101.5% after 28 days of storage at ?20 ℃, and 99.6% after 28 days of storage at 4 ℃, indicating that the sample has good uniformity and stability, and can be used as a positive quality control sample for the detection and quality control of Listeria monocytogenes. |
| |
| Keywords: | matrix-assisted laser desorption ionization time-of-flight mass spectrometry Listeria monocytogenes quality control samples for qualitative analysis homogeneity stability |
|
| 点击此处可从《乳业科学与技术》浏览原始摘要信息 |
| 点击此处可从《乳业科学与技术》下载免费的PDF全文 |
|
