| 水稻潜根线虫生防菌筛选、鉴定及其杀线虫活性研究 |
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| 引用本文： | 贾路明,莫爱素,莫意雪,吴海燕.水稻潜根线虫生防菌筛选、鉴定及其杀线虫活性研究[J].作物杂志,2023,39(2):238-1155. |
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| 作者姓名： | 贾路明 莫爱素 莫意雪 吴海燕 |
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| 作者单位： | 广西农业环境与农产品安全重点实验室/广西大学农学院，530004，广西南宁 |
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| 基金项目： | 国家自然科学基金(31460464) |
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| 摘 要： | 水稻潜根线虫在世界各稻区均普遍发生，给水稻生产造成一定的经济损失。从前期对根结线虫有较好毒杀活性的9个菌株中筛选出对水稻潜根线虫有很好毒杀效果的菌株。结果表明，2周发酵液2.5倍稀释液处理水稻潜根线虫，菌株ZW-1和XO-2对潜根线虫毒杀活性较强，72h的死亡率分别为93.7%和96.7%,LC50值分别为90.4451和76.3875μL/mL。根据形态学特征和rDNA-ITS序列分析，鉴定菌株ZW-1和XO-2分别为日本曲霉(Aspergillus japonicus)和黑曲霉(A.niger)。这些生防真菌菌株的发掘，为植物线虫病害的生物防治提供了新的微生物资源。
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| 关 键 词： | 水稻潜根线虫 生防菌 筛选 杀线虫活性 |
| 收稿时间： | 2021-08-04 |
Screening and Identification of Biocontrol Fungi against Hirschmanniella sp. and Analysis of Nematocidal Activity |
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| Jia Luming,Mo Aisu,Mo Yixue,Wu Haiyan.Screening and Identification of Biocontrol Fungi against Hirschmanniella sp. and Analysis of Nematocidal Activity[J].Crops,2023,39(2):238-1155. |
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| Authors: | Jia Luming Mo Aisu Mo Yixue Wu Haiyan |
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| Institution: | Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety/Agricultural College of Guangxi University, Nanning 530004, Guangxi, China |
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| Abstract: | Hirschmanniella sp. is common in all rice regions of the world, which causes some economic losses of rice production. From the nine strains with good virulence against root-knot nematodes, we selected the strains with good virulence against of rice latent root nematodes. The results showed that ZW-1 and XO-2 had high nematocidal activities against Hirschmanniella sp., and the mortality rate in 2.5×dilution for 72h were 93.7% and 96.7%, respectively, with LC50 values of 90.4451 and 76.3875μL/mL, respectively. Strains ZW-1 and XO-2 were identified as Aspergillus japonicus and A.niger, respectively, based on morphological and rDNA-ITS molecular analysis. The discovery of two biocontrol fungal strains provides new microbial resources for the biological control of plant nematode diseases. |
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| Keywords: | Hirschmanniella sp Biocontrol fungus Screening Nematocidal activity |
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