| 致病性猪链球菌2型的病原分离鉴定及毒力因子的PCR检测 |
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| 引用本文： | 胡杰,刘棋.致病性猪链球菌2型的病原分离鉴定及毒力因子的PCR检测[J].中国畜牧兽医,2009,36(1):49-52. |
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| 作者姓名： | 胡杰 刘棋 |
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| 作者单位： | 1.南京农业大学， 南京 210095； 2.广西区动物疫病预防控制中心， 南宁 530001 |
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| 摘 要： | 通过对广西某猪场急性死亡的猪进行病原分离，分离到革兰氏阳性球菌，用链球菌快速鉴定试剂条Rapid ID 32 Strep鉴定为猪链球菌2型；并对分离菌进行猪链球菌2型荚膜多糖抗原（cps2J）及其重要毒力因子溶菌酶释放蛋白（MRP）、细胞外蛋白因子（EF）和溶血素（SLY）的多重PCR检测，同时对cps2J基因进行序列测定，与GenBank发表的猪链球菌2型相比，同源性为98.8%，毒力因子MRP、EF和SLY检测均为阳性，证实广西某猪场急性死亡的猪为高毒力猪链球菌2型感染所致。动物试验中，该菌可引起小白鼠部分死亡，家兔体温最高升至40.3℃，最后败血而死。
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| 关 键 词： | 猪链球菌2型 病原分离 毒力因子 PCR |
Isolation and Identification on Pathogens of Swine Streptococcosis and PCR Assays for Detection of Virulence Factors of Streptococcus suis Serotype 2 |
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| HU Jie,LIU Qi.Isolation and Identification on Pathogens of Swine Streptococcosis and PCR Assays for Detection of Virulence Factors of Streptococcus suis Serotype 2[J].China Animal Husbandry & Veterinary Medicine,2009,36(1):49-52. |
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| Authors: | HU Jie LIU Qi |
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| Institution: | 1.Nanjing Agriculture University, Nanjing 210095, China；2.Guangxi Animal Center for Disease Control and Prevention, Nanning 530001, China |
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| Abstract: | Gram-postive bacterium,identificated with Streptococcus suis serotype 2 by Rapid ID 32 Strep reagent of Streptococcus suis,was isolated in Guangxi.PCR assays were performed for detection of cps2J and three virulence factors,muramidase-relseased protein(MRP),extracelluar factor(EF) and suilysin gene(SLY) of Streptococcus suis.The sequence analysis revealed 98.8% homology to that of cps2J in GenBank.Virulence factors of Streptococcus suis serotype 2 were sly+mrp+ef+,which indicated that the cause of acute dea... |
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| Keywords: | PCR |
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