| 氮离子注入井冈霉素产生菌诱变高产菌株的研究 |
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| 引用本文： | 赵南,吴冬兰,唐洪啟,毛玉华,贺婷,张文宣.氮离子注入井冈霉素产生菌诱变高产菌株的研究[J].江西农业学报,2010,22(4):122-123. |
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| 作者姓名： | 赵南 吴冬兰 唐洪啟 毛玉华 贺婷 张文宣 |
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| 作者单位： | 江西核工业瑞丰生化有限责任公司,江西,新干,331307 |
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| 摘 要： | 探索了氮离子注入技术在井冈霉素产生菌高产菌株诱变中的应用。向井冈霉素产生菌注入能量10 keV、剂量15×1013个/cm2氮离子实施诱变,再生培养后单菌落接斜面上摇床进行效价测定,筛选高产菌株。结果获得诱变菌株9275#,其有效A组分含量较出发菌株提高33.52%,且具有高的遗传稳定性。
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| 关 键 词： | 氮离子注入 井冈霉素产生菌 诱变 高产菌株 |
Study on Mutation of High-yielding Strain by Injecting Nitrogen Ion into Validamycin-producing Strain |
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| ZHAO Nan,WU Dong-lan,TANG Hong-qi,MAO Yu-hua,HE Ting,ZHANG Wen-xuan.Study on Mutation of High-yielding Strain by Injecting Nitrogen Ion into Validamycin-producing Strain[J].Acta Agriculturae Jiangxi,2010,22(4):122-123. |
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| Authors: | ZHAO Nan WU Dong-lan TANG Hong-qi MAO Yu-hua HE Ting ZHANG Wen-xuan |
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| Institution: | Jiangxi Nuclear Industrial Ruifeng Biochemical Limited Liability Company/a>;Xin'gan 331307/a>;China |
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| Abstract: | The application of nitrogen ion beam injection technique in the mutation of high-yielding validamycin-producing strain was explored.After injecting 15×1013 nitrogen ions per metric centimeter with the intensity of 10 keV into validamycin-producing strain for mutation,the regenerated single-colonies were inoculated in shake flasks for the selecting of high-yielding strains.A mutative strain 9275# was obtained,the content of effective component A in strain 9275# was 33.52% higher than that in the original strain,and 9275# had high genetic stability. |
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| Keywords: | Injection of nitrogen ion beam Validamycin-producing strain Mutation High-yielding strain |
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