| 浦竹仔红色秆变异个体的rDNA ITS序列及系统发育研究 |
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| 引用本文： | 王娟,孙浩,彭桂莎,王明悦,熊智,张兴波,孙茂盛,杨宇明.浦竹仔红色秆变异个体的rDNA ITS序列及系统发育研究[J].西部林业科学,2012(1):1-6. |
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| 作者姓名： | 王娟 孙浩 彭桂莎 王明悦 熊智 张兴波 孙茂盛 杨宇明 |
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| 作者单位： | 西南林业大学国家林业局西南生物多样性保育重点实验室;云南兴云园艺有限公司;云南省林业科学院 |
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| 基金项目： | 国家林业局948项目(2008-4-30)竹子重要性状的基因工程研究技术引进;云南省自然科学基金面上项目(2009CD073)资助研究;云南省中青年学术技术带头人后备人才培养项目(2010CI016)资助研究 |
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| 摘 要： | 通过对浦竹仔红秆变异个体基因组DNA的提取、rDNA ITS片段的扩增、回收及测序,分析了其rDNA ITS序列,构建了系统发育树。结果表明,供试竹种与Genbank中记录的浦竹仔ITS全长序列相似度为99%,与同属的中华大节竹、酸竹属的酸竹、少穗竹属的少穗竹亲缘关系较近,其遗传距离为0.007和0.010。RNA二级结构模拟分析显示,各参试竹种之间5.8s RNA变异极微小,主要的遗传变异均集中在ITS1-ITS2非编码序列中。由此可以推论该竹种产花色苷性状并非种属差异导致,为样本收集地环境因子诱发基因突变所致。
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| 关 键 词： | 浦竹仔 变异个体 rDNA ITS序列 系统发育 |
Phylogenetic Study on Anthocyanin Produced Mutant of Indosasa hispida Based on rDNA ITS Sequences |
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| WANG Juan,SUN Hao,PENG Gui-sha,WANG Ming-yue,XIONG Zhi,ZHANG Xing-bo,SUN Mao-sheng,YANG Yu-ming.Phylogenetic Study on Anthocyanin Produced Mutant of Indosasa hispida Based on rDNA ITS Sequences[J].Journal of West China Forestry Science,2012(1):1-6. |
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| Authors: | WANG Juan SUN Hao PENG Gui-sha WANG Ming-yue XIONG Zhi ZHANG Xing-bo SUN Mao-sheng YANG Yu-ming |
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| Institution: | 1.Key Laboratory of Biodiversity Conservation in Southwest of China,State Forestry Administration,Southwest Forestry University, Kunming Yunnan 650224,P.R.China;2.Yunnan Academy of Forestry,Kunming Yunnan 650201,P.R.China; 3.Yunnan Xingyun Gardening Co.Ltd.,Pu’er Yunnan 665000,P.R.China) |
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| Abstract: | Through DNA extraction,PCR amplification and DNA sequencing,the internal transcribed spacers(ITS) sequence of anthocyanin produced mutant of Indosasa hispida were analyzed and the phylogenetic tree was established.The results indicated that the ITS sequence similarity between the tested materials and the record of Indosasa hispida was 99 %,anthocyanin produced mutant had close genetic relationship with Indosasa sinica,Acidosasa purpurea and Oligostachyum sulcatum,with the genetic distance of 0.007 and 0.010 respectively.RNA secondary structure simulation analysis showed the genetic variation in 5.8 sRNA was extremely small,the main genetic variation were in ITS1-ITS2 noncoding sequence.The results therefore implied that the anthocyanin produced character was not the genus or species difference,but caused by environmental mutagenesis. |
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| Keywords: | Indosasa hispida individual variation rDNA ITS sequence phylogenesis |
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