| 油菜几丁质酶的特点及其与抗菌核病的关系 |
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| 引用本文： | 张学昆,李加纳,唐章林,谌利,陈云坪,陈洪源,郭益红.油菜几丁质酶的特点及其与抗菌核病的关系[J].西南农业大学学报,2001,23(3):208-210,215. |
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| 作者姓名： | 张学昆 李加纳 唐章林 谌利 陈云坪 陈洪源 郭益红 |
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| 作者单位： | 西南农业大学农学系，重庆北碚400716 |
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| 基金项目： | 四川省“九·五”育种攻关项目：油菜高产优质抗病高效育种方法研究子专题 |
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| 摘 要： | 通过测定甘蓝型油菜西农长角几丁质酶对底物的特异性等性质，结果表明，几丁质酶以脱乙酰化几丁质7B（乙酰化程度为30％）为底物时活性最高，而对8B，9B，10B（乙酰化程度为20％，10％，0％）和菌核菌细胞壁的活性较低。几丁质酶活性的最适宜反应温度为30℃-40℃中，并在25℃以下时活性大大降低，这与菌核菌生长和入侵的最适温度15℃-25℃不一致。西农长角的种子中几丁质酶的活性很强，发芽后逐渐下降，到第5天时开始提高。研究还发现，不同类型和抗性油菜材料的几丁质酶活性和对菌核菌的抗性间存在相关关系，相关系数达显著水平，这表明几丁质酶在提高油菜的抗菌核病能力起着重要的作用。
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| 关 键 词： | 油菜 几丁质酶 菌核病 抗病性 |
| 文章编号： | 1000-2642(2001)03-0208-03 |
SOME CHARACTERISTICS OF CHITINASE OF RAPESEED AND ITS RELATION SHIP WITH RESISTANCE TO SCLEROTINIA SCLEROTIORUM |
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| ZHANG Xue-kun,LI Jia-na,TANG Zhang-lin,CHEN Li,CHEN Yun-ping,CHEN Hong-yuan,GUO Yi-hong.SOME CHARACTERISTICS OF CHITINASE OF RAPESEED AND ITS RELATION SHIP WITH RESISTANCE TO SCLEROTINIA SCLEROTIORUM[J].Journal of Southwest Agricultural University,2001,23(3):208-210,215. |
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| Authors: | ZHANG Xue-kun LI Jia-na TANG Zhang-lin CHEN Li CHEN Yun-ping CHEN Hong-yuan GUO Yi-hong |
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| Abstract: | Four partially N-acetylated chitosans, i.e. 7B, 8B, 9B and 10B with a degree acetylation of 70%, 80%, 90% and 100%, respectively, and the cell wall of Sclerotinia sclerotiorum (CW)were used as substrates to test the possible specificity of the chitinase of the rapeseed (Brassica napus) cv. Xinong Changjiao to the substrates. The results showed that the chitinase produced the highest activity to chitisan 7B and lower activity to 8B, 9B, 10B and CW, suggesting that the chitinase has no specificity to the cell wall chitosan of S. sclerotiorum. Another study with 11rapeseed varieties or lines indicated that the activity of the chitinase of different rapeseed genotypes showed a significant negative correlation with their disease scores, suggesting that chitinase may be of great significance in improving the resistance to S. sclerotiorum in rapeseed. |
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| Keywords: | rapeseed chitinase Sclerotinia sclerotiorum |
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