Establishment and Optimization of a Rapid Molecular Marking Method for Developing CAPS Molecular Markers in Soybean |
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| Authors: | SHU Yongjun LI Yong WU Nalahu ZHU Yanming |
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| Institution: | College of Life Sciences, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | In this study, a rapid molecular marking method, gspCAPS (genome sequence pool CAPS), was established to develop soybean CAPS markers. The bioinformatics tools were executed for analyzing the sequences of soybean SNP sites and selecting the appropriate restriction endonucleases. Meanwhile, DNA samples were mixed, and the primers were sequentially screened by PCR and enzyme digestion procedures. To evaluate this method, 61 pairs of primers were designed and polymorphisms were detected in nine soybean varieties. The results showed that the method remarkably improved its efficiency and characterized with advantages such as high efficiency, timesaving and low cost, which had a widespread prospect for applying in the future. |
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| Keywords: | soybean gspCAPS bioinformatics optimization |
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