| 卷丹百合黑斑病病原鉴定及生物学特性研究 |
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| 引用本文： | 吴金平,丁自立,刘晓艳,郭凤领,焦忠久,邱正明.卷丹百合黑斑病病原鉴定及生物学特性研究[J].农业科学与技术,2014(11):1879-1882. |
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| 作者姓名： | 吴金平 丁自立 刘晓艳 郭凤领 焦忠久 邱正明 |
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| 作者单位： | 湖北省农业科学院,湖北武汉,430064 |
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| 基金项目： | 农业部现代农业产业技术体系项目(nycytx-35-02-06)；湖北省农业重点应用基础研究项目(2012DBA07)。Supported by Modern Agricultural Industry Technology System Project of Ministry of Agriculture，Key Program for Applied Basic Research of Agriculture of Hubei Provincial Science and Technology Plan Project |
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| 摘 要： | 随着卷丹百合种植面积的扩大，卷丹百合叶部真菌性病害也越来越严重，尤其在2014年6~7月份，由于降水偏多，卷丹百合叶部病害大发生，6月中旬重病田卷丹百合枯死株率达50%~70%，部分田块卷丹百合地上部全部枯死。从卷丹百合发病叶片分离得到病原菌，进行致病性鉴定、孢子形态观察、18S rDNA序列分析，生物学特性研究，结果表明，该病原菌为半知菌链格孢（ Alternaria alternata）。该菌菌丝生长致死温度为55℃；适宜 pH为6~7；在供试的几种碳、氮源中，最适的碳源是麦芽糖，最适的氮源是酵母。
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| 关 键 词： | 卷丹百合 黑斑病 生物学特性 分子鉴定 |
Identification and Biological Characteristics of Blank Spot Pathogen from Lilium lancifolium |
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| Jinping WU,Zili DING,Xiaoyan LIU,Fengling GUO,Zhongjiu JIAO,Zhengming QIU.Identification and Biological Characteristics of Blank Spot Pathogen from Lilium lancifolium[J].Agricultural Science ＆ Technology,2014(11):1879-1882. |
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| Authors: | Jinping WU Zili DING Xiaoyan LIU Fengling GUO Zhongjiu JIAO Zhengming QIU |
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| Institution: | (Hubei Academy of Agricultural Sciences, Wuhan 430064, China) |
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| Abstract: | With the increasing planting area of Lilium lancifolium, the leaf fungal dis-ease of L. lancifolium is becoming more and more serious. In June and July of 2014, the excessive rainfal leads to the serious outbreak of leaf disease of L. lanci-folium. In mid-June, the wilting rate of L. lancifolium in seriously-infected field was even up to 50%-70%. In some fields, the shoots of L. lancifolium even al wilted. The pathogen was isolated from the infected leaves of L. lancifolium. Its pathogenici-ty, spore morphology, 18S rDNA sequence and biological characteristics were stud-ied. The results showed the isolated pathogen was Alternaria alternate. The lethal temperature of mycelial growth was 55 ℃. The optimum pH was 6-7. Among the tested carbon sources and nitrogen sources, the optimum carbon source was mal-tose, and the optimum nitrogen source was yeast extract. |
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| Keywords: | Lilium lancifolium Black spot Biological characteristic Molecular identifi-cation |
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