Uptake of 15 trace elements in arbuscular mycorrhizal marigold: Measured by the multitracer technique |
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| Authors: | Hiroyuki Suzuki Hiroshi Kumagai Kunio Oohashi Kazuyuki Inubushi Shuichi Enomoto |
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| Institution: | 1. Radioisotope Research Center , Chiba University , Chiba , 263-8522 , Japan;2. Faculty of Pharmaceutical Sciences , Chiba University , Chiba , 263-8522 , Japan , Matsudo , 271-8510 , Japan;3. The Institute of Physical and Chemical Research (RIKEN) , Wako , 351-0198 , Japan |
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| Abstract: | The effect of arbuscular mycorrhizal (AM) colonization on the uptake of trace elements in marigold (Tagetes patula L.) was studied using a multitracer consisting of radionuclides of 7Be, 22Na, 46Sc, 51Cr, 54Mn, 59Fe, 56Co, 65Zn, 74As, 75Se, 83Rb, 85Sr, 88Y, 88Zr, and 95mTc. Marigold plants were grown under controlled environmental conditions in sand culture either without mycorrhizas or in association with an AM fungus, Glomus etunicatum. The multitracer was applied to the pot, and plants were harvested at 7 and 21 d after tracer application. We found that the uptake of 7Be, 22Na, 51Cr, 59Fe, 65Zn, and 95mTc was higher in the mycorrhizal marigolds than in the non-mycorrhizal ones, while that of 46Sc, 56Co, 83Rb, and 85Sr was lower in the mycorrhizal marigolds than in the non-mycorrhizal ones. Thus, the multitracer technique enabled to analyze the uptake of various elements by plant simultaneously. It is suggested that this technique could be used to analyze the effects of AM colonization on the uptake of trace elements by plant. |
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| Keywords: | endophytic diazotrophs Japanese sugarcane cultivar 15N dilution method natural 15N abundance method nitrogen fixation |
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