A rice FRD3-like (OsFRDL1) gene is expressed in the cells involved in long-distance transport |
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| Authors: | Haruhiko Inoue Daichi Mizuno Michiko Takahashi Hiromi Nakanishi Satoshi Mori Naoko K Nishizawa |
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| Institution: | 1. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo, 113-8657, Japan;2. Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo, 113-8657, Japan;3. Department of Global Agricultural Sciences, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo, 113-8657, Japan;4. Core Research for Evolutional Science and Technology (CREST) , Japan Science and Technology Corporation , Japan |
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| Abstract: | We identified four putative AtFRD3-like genes (OsFRDL) in the rice genome that exhibited 39.1 to 56.7% amino acid sequence similarities to Arabidopsis FRD3. Of these, we cloned three OsFRDL genes from a cDNA library prepared from iron-deficient rice roots: OsFRDL1, OsFRDL2, and OsFRDL3. OsFRDL1 was expressed weakly in Fe-sufficient roots, and slight expression was induced in the roots of Fe-deficient plants. OsFRDL2 was expressed constitutively in both roots and leaves, and Fe deficiency reduced its expression in leaves. OsFRDL3 was expressed in leaves, but not in roots; Fe deficiency induced slight expression in leaves. An OsFRDL1-sGFP fusion protein was localized in the plasma membrane in onion epidermal cells. The promoter GUS analysis showed that OsFRDL1 was localized in the cells involved in long-distance transport, in both Fe-sufficient and Fe-deficient plants. Furthermore, OsFRDL1 expression was observed during the reproductive stage. These results suggest that OsFRDL1 is a transporter that resides in the plasma membrane of cells involved in long-distant transport. |
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| Keywords: | companion cell iron MATE nicotianamine phloem |
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