A simple and efficient method for the long-term preservation of plant cell suspension cultures |
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| Authors: | Anne-Marie Boisson Elisabeth Gout Richard Bligny and Corinne Rivasseau |
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| Institution: | (1) Commissariat ? l’Energie Atomique, Laboratoire de Physiologie Cellulaire V?g?tale, Unit? Mixte de Recherche 5168 CNRS, UJF, INRA, CEA, institut de Recherche en Technologies et Sciences pour le Vivant, F-38054 Grenoble, France |
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| Abstract: | Background The repeated weekly subculture of plant cell suspension is labour intensive and increases the risk of variation from parental
cells lines. Most of the procedures to preserve cultures are based on controlled freezing/thawing and storage in liquid nitrogen.
However, cells viability after unfreezing is uncertain. The long-term storage and regeneration of plant cell cultures remains
a priority. |
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