| 黄颡鱼仔稚鱼发育过程中的胰蛋白酶原基因表达 |
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| 引用本文： | 高 超,杨瑞斌,胡文彪,范锦桧.黄颡鱼仔稚鱼发育过程中的胰蛋白酶原基因表达[J].华中农业大学学报,2012,31(2):227-230. |
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| 作者姓名： | 高 超 杨瑞斌 胡文彪 范锦桧 |
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| 作者单位： | 1. 华中农业大学水产学院,武汉,430070
2. 福建省大田县奇韬镇水产站,大田,366108 |
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| 基金项目： | 广东省科技厅项目（2010B090500026） |
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| 摘 要： | 采用RT-PCR和实时荧光定量PCR方法,得到黄颡鱼(Pelteobagrusf ulvidraco)消化系统胰蛋白酶原基因cDNA,并且对其早期发育过程中仔稚鱼(1～3日龄)消化系统胰蛋白酶原基因表达进行检测和定量。结果表明,黄颡鱼消化系统中胰蛋白酶原的基因表达起始于1日龄,1～6日龄表达量快速增加、6～23日龄表达量呈起伏式增加、23～30日龄表达量显著增加。该结果同时表明使用实时荧光定量PCR的方法检测黄颡鱼胰蛋白酶原基因的表达量,能反映其肠道消化功能在发育过程中的变化。建议黄颡鱼仔稚鱼转饵时间在23日龄后。
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| 关 键 词： | 黄颡鱼 仔稚鱼 胰蛋白酶原 基因表达 荧光定量PCR |
| 收稿时间： | 2011/5/28 0:00:00 |
Trypsinogen gene expression of yellow catfish Pelteobagrus fulvidraco larvae and juvenile |
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| GAO Chao
,
YANG Rui-bin
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HU Wen-biao
,
FAN Jin-hui.Trypsinogen gene expression of yellow catfish Pelteobagrus fulvidraco larvae and juvenile[J].Journal of Huazhong Agricultural University,2012,31(2):227-230. |
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| Authors: | GAO Chao YANG Rui-bin HU Wen-biao FAN Jin-hui |
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| Institution: | 1.College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China; 2.The Fishery Technical Service Station of Qitao,Datian 366108,China |
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| Abstract: | The first-strand cDNA sequence encoding pancreatic trypsinogen(TRY) gene was isolated from yellow catfish(Pelteobagrus fulvidraco) larvae(1-30 dph,day post-hatching) by RT-PCR,and using real-time PCR to detected and quantitated the trypsinogen gene expression.The results showed that the onset of trypsinogen gene expression in P.fulvidraco larvae was 1 dph and the trypsinogen gene expression increased significantly from 1 to 6 dph,increased fluctuantly from 6 to 23 dph and rose sharply from 23 to 30 dph.The experimental results demonstrated that the detection and quantitation of trypsinogen gene expression in P.fulvidraco larvae would accurately reflect the process that digestive functioning developed in intestinal tract.And we suggested that weaning time of P.fulvidraco larvae would be much better after 23 dph. |
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| Keywords: | Pelteobagrus fulvidraco larvae trypsinogen gene expression real-time PCR |
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