| 褐牙鲆消化道内分泌细胞免疫组织化学的定位 |
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| 引用本文： | 王寿昆,李君秋,林建斌,林树根.褐牙鲆消化道内分泌细胞免疫组织化学的定位[J].福建农林大学学报(自然科学版),2007,36(2):171-175. |
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| 作者姓名： | 王寿昆 李君秋 林建斌 林树根 |
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| 作者单位： | 1. 福建农林大学动物科学学院,福建,福州,350002
2. 福建省淡水水产研究所,福建,福州,350102 |
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| 摘 要： | 应用过氧化物酶标记的链霉亲和素免疫组织化学技术,用抗5-羟色胺(5-HT)、生长抑素(SST)、胃泌素(Gas)、胰高血糖素(G lu)等4种抗哺乳动物血清对褐牙鲆消化道内分泌细胞进行免疫组织化学定位的研究.结果表明:5-HT免疫阳性细胞在消化道各段均有分布,胃各部分布较多,食管和肠管分布较少;SST在胃贲门部和胃体部数量较多,胃幽门部较少,食管和肠管各段均未检出;Gas分布于胃幽门部和前肠、中肠、后肠,从胃幽门部到后肠呈递减分布趋势,食管、胃贲门部和胃体部均未检出;G lu免疫阳性细胞分布于前肠和中肠,其余各段均未检出.
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| 关 键 词： | 褐牙鲆 消化道 内分泌细胞 免疫组织化学 |
| 文章编号： | 1671-5470(2007)02-0171-05 |
| 修稿时间： | 2006-04-26 |
Localization on immunohistochemistry of endocrine cells in the digestive tract of Paralichthys olivaceus |
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| WANG Shou-kun,LI Jun-qiu,LIN Jian-bing,LIN Shu-gen.Localization on immunohistochemistry of endocrine cells in the digestive tract of Paralichthys olivaceus[J].Journal of Fujian Agricultural and Forestry University,2007,36(2):171-175. |
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| Authors: | WANG Shou-kun LI Jun-qiu LIN Jian-bing LIN Shu-gen |
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| Institution: | 1. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China; 2. Fujian Institute of Aquatic Product in Freshwater, Fuzhou, Fujian 350102, China |
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| Abstract: | The distribution of endocrine cells in Paralichthys olivaceus alimentary canal was studied through immunohistochemistry,using anti-serum of 5hydroxytryptamine(5-HT),somatostatin(SST),gastrin(Gas) and glucagon(Glu).It is showed that immunological active endocrine cells of 5-HT in every section of alimentary canal were more in stomach and few in oesophagus and intestines.SST-cells were found more in stomachus cardiacus,stomachus fundus,few in stomachus pyloricus,but not found in oesophagus and intestine.Gas-cells were found in stomachus pyloricus,foregut,midgut and hidgut,the quantity decreased gradually from stomachus pyloricus to hidgud, but not found in oesophagus,stomachus cardiacus,stomachus fundus.Glucells were found in foregut and midgut,not found in other parts of alimentary canal. |
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| Keywords: | Paralichthys olivaceus digestive tract endocrine cell immunohistochemistry |
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