| 水稻稻瘟病菌有性世代形成条件的优化 |
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| 引用本文： | 张国珍,马秋娟,彭友良.水稻稻瘟病菌有性世代形成条件的优化[J].植物病理学报,2002,32(2):121-126. |
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| 作者姓名： | 张国珍 马秋娟 彭友良 |
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| 作者单位： | 中国农业大学植物保护学院, 北京 100094 |
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| 基金项目： | 国家自然科学基金重点项目 (39730 2 0 )，云南省省校植物病理学实验室共建项目资助 |
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| 摘 要： | 本研究通过改变燕麦片番茄培养基中的一些成分,包括番茄成熟度、pH值、钙的添加等,探讨了稻瘟病菌有性世代形成的适宜条件。结果表明:选用成熟番茄的榨汁作为培养基的主要组分之一,调节培养基pH值在5.5左右,并添加0.1% CaCO3能显著提高子囊壳形成的数量、促进子囊和子囊孢子的发育、提高子囊孢子的成活率。在上述培养基上进行稻瘟病菌的有性杂交,并在子囊壳开始出现后7~18d内分离子囊孢子,其成活率可高达85.5%。
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| 关 键 词： | 稻瘟病菌 培养基 有性世代 子囊孢子分离 |
| 文章编号： | 0412-0914(2002)02-0121-06 |
| 修稿时间： | 2001年3月28日 |
OPTIMIZATION FOR PRODUCING PERFECT STAGE OF RICE BLAST FUNGUS Magnaporthe grisea |
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| ZHANG Guo zhen,MA Qiu juan,PENG You liang.OPTIMIZATION FOR PRODUCING PERFECT STAGE OF RICE BLAST FUNGUS Magnaporthe grisea[J].Acta Phytopathologica Sinica,2002,32(2):121-126. |
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| Authors: | ZHANG Guo zhen MA Qiu juan PENG You liang |
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| Institution: | China Agricultural University, Beijing 100094, China |
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| Abstract: | Optimization for the perfect stage production of Magnaporthe grisea in vitro were investigated by using juice of tomato with different maturity, changing pH and adding CaCO 3 in the oatmeal tomato agar medium. Results showed that the number of perithecia and ascospores and the percentage of viable ascospores were markedly increased when juice of mature tomato was used, pH was adjusted to around 5.5 and 0.1% CaCO 3 was added in the medium. Additionally, the optimal time for isolating ascospores is about 7 18 days after perithecia formation. During this period survival percentage of ascospores can reach up to 85.5%. |
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| Keywords: | Magnaporthe grisea medium perfect stage isolation of ascospores |
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