| 采用吡啶三氧化硫制备硫酸化丝素及其抗凝血性能检测 |
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| 引用本文： | 李圣春,侯春春,徐水.采用吡啶三氧化硫制备硫酸化丝素及其抗凝血性能检测[J].蚕业科学,2010,36(3):553-557. |
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| 作者姓名： | 李圣春 侯春春 徐水 |
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| 作者单位： | 西南大学生物技术学院,重庆,400716;西南大学生物技术学院,重庆,400716;西南大学生物技术学院,重庆,400716 |
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| 摘 要： | 硫酸化丝素是一种新型的蛋白基抗凝血医用材料。以丝素蛋白和吡啶三氧化硫为原料制备可溶性硫酸化丝素。对硫酸化处理不同时间获得的丝素蛋白样品进行红外光谱检测,结果表明用吡啶三氧化硫处理可溶性丝素蛋白2h以上可以将SO42-接枝到丝素蛋白上,当处理时间达到6h后含硫量达到最大值(1.26mmol/g)。将此硫酸化丝素配制成质量浓度为0.8mg/mL的溶液,其抗凝血效果能达到0.06mg/mL肝素钠的抗凝血水平,使血液在1h内不会凝固。该制备方法简单易行,得到的硫酸化丝素的抗凝血效果良好,有望进一步开发成为新型医用抗凝血材料。
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| 关 键 词： | 丝素蛋白 吡啶三氧化硫 硫酸化丝素 抗凝血 |
Preparation and Anticoagulation Test of Sulfated Silk Fibroin with Pyridine Sulfur Trioxide |
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| LI Sheng-Chun,HOU Chun-Chun,XU Shui.Preparation and Anticoagulation Test of Sulfated Silk Fibroin with Pyridine Sulfur Trioxide[J].Acta Sericologica Sinica,2010,36(3):553-557. |
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| Authors: | LI Sheng-Chun HOU Chun-Chun XU Shui |
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| Abstract: | Sulfated silk fibroin is a new type protein-based anticoagulant medicinal material. In present study,silk fibroin and pyridine sulfur trioxide were used to prepare soluble sulfated silk fibroin. Inspection of various sulfated silk fibroin samples by infrared spectrometry showed that SO42could be grafted onto silk fibroin when the soluble silk fibroin was treated with pyridine sulfur trioxide for 2 h or longer. When the treatment time was prolonged to 6 h,the sulfur content reached the maximum level (1. 26 mmol/g). After this sulfated silk fibroin was prepared into a solution with 0. 8 mg /mL mass concentration,its anticoagulant effect achieved the same anticoagulant level with 0. 06 mg /mL heparin sodium, meaning that the blood would not coagulate within 1 h. This method is simple and easy to operate. The obtained sulfated silk fibroin has satisfactory anticoagulant effect,being a promising new type anticoagulant medicinal material for future development. |
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| Keywords: | Silk fibroin Pyridine sulfur trioxide Sulfated fibroin Anticoagulation |
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