| 黄瓜果实苦味Bt基因的AFLP分子标记 |
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| 引用本文： | 顾兴芳,张素勤,张圣平.黄瓜果实苦味Bt基因的AFLP分子标记[J].园艺学报,2006,33(1):140-142. |
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| 作者姓名： | 顾兴芳 张素勤 张圣平 |
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| 作者单位： | ( 中国农业科学院蔬菜花卉研究所, 北京100081; 2 西北农林科技大学园艺学院, 杨凌712100) |
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| 基金项目： | 国家‘863’计划项目(2002AA207012、2001AA241121)，农业部蔬菜遗传与生理重点开放实验室资助项目 |
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| 摘 要： | 运用AFLP技术, 采用集群分析法(BSA) 进行与黄瓜果实苦味基因连锁的分子标记的研究,找到了与苦味基因连锁的两个显性AFLP标记: E23M662101和E25M652213。这两个标记与Bt基因的遗传距离分别为5 cM和4 cM, 分别位于Bt基因的两侧。该标记可用于无苦味黄瓜的分子标记辅助育种。
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| 关 键 词： | 黄瓜 果实苦味Bt基因 AFLP标记 |
| 文章编号： | 0513-353X（2006）01-0140-03 |
| 收稿时间： | 2005-06-30 |
| 修稿时间： | 2005-06-302005-07-28 |
The AFLP Markers Linked with the Bitter Fruit Gene (Bt) in Cucumber |
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| Gu Xingfang,Zhang Suqin,Zhang Shengping.The AFLP Markers Linked with the Bitter Fruit Gene (Bt) in Cucumber[J].Acta Horticulturae Sinica,2006,33(1):140-142. |
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| Authors: | Gu Xingfang Zhang Suqin Zhang Shengping |
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| Institution: | (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China; College of Horticulture, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China) |
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| Abstract: | AFLP biotechnology was applied to identify the markers linked to Bt gene based on BSA method.Two dominant AFLP markers E23M66-101 and E25M65-213 were screened firstly in home and abroad.The bitter fruit F_2 plants possessed 101 bp and 213 bp fragments,but the bitterfree F_2 plants not.The genetic distance of E23M66-101 and E25M65-213 was 5 cM and 4 cM respectively,and two markers were two sides of Bt gene.The AFLP markers can be used for marker-assisted selection. |
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| Keywords: | Cucumber Bt gene AFLP marker |
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