Comparative analysis of <Emphasis Type="Italic">Argonaute</Emphasis> gene sequences in bananas (<Emphasis Type="Italic">Musa</Emphasis> sp.) shows conserved species-specific Ago-7 PIWI domains |
| |
| Authors: | Chee?How?Teo Han?Pin?Pui Rofina?Yasmin?Othman Email author" target="_blank">Jennifer?Ann?HarikrishnaEmail author |
| |
| Institution: | (1) Centre for Research in Biotechnology for Agriculture (CEBAR) & Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; |
| |
| Abstract: | The Argonaute proteins are key components in the effector complex of RNA silencing pathways which interact with small RNAs
to mediate sequence specific silencing of nucleic acid targets. In plants, these proteins are involved in a diversity of biological
roles such as antiviral defence, heterochromatin regulation and in the regulation of growth and development. This report describes
the study of Argonaute gene sequences in bananas which are an important food staple for many developing nations. This study successfully isolated
AGO7-specific PIWI domain genomic sequences from 12 diploid Musa species of three Musa sections and also from Ensete. The Musa AGO7-specific PIWI domain sequences showed the highest similarity to rice AGO7/SHOOTLESS4 with an average amino acid identity
of 78%. Phylogenetic analysis of the Musa sequences revealed phylogenetic grouping that agrees fairly well with the present knowledge of the taxonomic classification
of Musa species. In addition, this study estimated that there are at least 15 Argonaute genes or loci containing PIWI domain sequences in the genome of Musa acuminata ssp. malaccensis. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
