| 油茶组织培养繁殖技术初步研究 |
| |
| 引用本文： | 唐国涛,张汉永,黄锦荣,蔡梅玲,朱昔娇,罗万业,邵萍,吴明芳.油茶组织培养繁殖技术初步研究[J].广东林业科技,2014,30(3):25-29. |
| |
| 作者姓名： | 唐国涛 张汉永 黄锦荣 蔡梅玲 朱昔娇 罗万业 邵萍 吴明芳 |
| |
| 作者单位： | 梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011,梅州市林业科学研究所 514011 |
| |
| 基金项目： | 广东省梅州市科技局农业公关项目“油茶优良无性系组培快繁技术研究” |
| |
| 摘 要： | 以油茶（Camellia oleifera）种子胚芽和2a生扦插苗带侧芽的嫩枝作外植体进行组织培养技术研究。结果表明：适合油茶种子胚芽诱导的培养基为MS＋6-BA2．0mg／L．4-NAA0．2mg／L＋GA31．0mg／L＋蔗糖30g／L＋卡拉胶6．5g／L，适宜的增殖培养基为MS＋6-BA1．5mg／L＋NAA0．1mg／L＋GA31．0mg／L＋蔗糖30g／L＋卡拉胶6．5g／L，增殖倍数为3．50；适合油茶不定芽诱导的培养基为MS＋6-BA1．5mg／L＋IAA2．0mg／L＋GA，1．0mg／L＋蔗糖30g／L＋卡拉胶6．5g／L，适宜的增殖培养基为MS＋6-BA2．0mg／L＋IAA1．0mg／L＋GA，1．0mg／L＋蔗糖30g／L＋卡拉胶6．5g／L，增殖倍数为3．96。木质化程度已较高的油茶组培茎芽适宜的生根基质为细沙，生根率达95％。
|
| 关 键 词： | 油茶 组织培养 繁殖技术 |
| 收稿时间： | 2014/3/18 0:00:00 |
| 修稿时间： | 2014/5/20 0:00:00 |
A Preliminary Study on Tissue Culture Technology of Camellia oleifera (Forestry Research Institute of Meizhou city 514011) |
| |
| Tang Guo-tao,and.A Preliminary Study on Tissue Culture Technology of Camellia oleifera (Forestry Research Institute of Meizhou city 514011)[J].Forestry Science and Technology of Guangdong Province,2014,30(3):25-29. |
| |
| Authors: | Tang Guo-tao and |
| |
| Institution: | (Forestry Research Institute of Meizhou, Meizhou, Guangdong 514011, China) |
| |
| Abstract: | Seed embryo or 2-year-old cuttings sprout shooting of Camellia oleifera were used as explants for tissue culture propagation technology research. The results were as follows ： the suitable induced medium of C. oleifera seed embryo was MS ＋ 6-BA 2.0 mg/L ＋ NAA 0.2 mg/L ＋ GA3 1.0 mg/L ＋ sucrose 30 g/L ＋ carrageenan 6.5 g/L, and its suitable proliferation medium was MS ＋ 6-BA 1.5 mg/L ＋ NAA 0.1 rng/L ＋ GA3 1.0 mg/L ＋ su- crose 30 g/L ＋ carrageenan 6.5 g/L, the multiplication rate was 3.50. The suitable induced medium of axillary bud was MS ＋ 6-BA 1.5 mg/L ＋ IAA 2.0 mg/L ＋ GA3 1.0 mg/L ＋ sucrose 30 g/L ＋ carrageenan 6.5 g/L, and its suitable proliferation medium was MS ＋ 6-BA 2.0 mg/L ＋ IAA 1.0 mg/L ＋ GA3 1.0 mg/L ＋ sucrose 30 g/L ＋ carrageenan 6.5 g/L, the multiplication rate was 3.96. The fine sand was a suitable matrixes for rooting of highly lignification shoots, the rooting rate was 95%. |
| |
| Keywords: | Camellia oleifera tissue culture breeding technology |
| 本文献已被 CNKI 维普 等数据库收录！ |
| 点击此处可从《广东林业科技》浏览原始摘要信息 |
| 点击此处可从《广东林业科技》下载免费的PDF全文 |
|
