Increased induction of embryogenesis and regeneration in anther cultures ofSolanum tuberosum L. |
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| Authors: | Lars B Johansson |
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| Institution: | (1) Department of Plant Breeding, Swedish University of Agricultural Sciences, Box 7003, S-75007 Uppsala, Sweden |
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| Abstract: | Summary A new method is presented for anther culture. Anthers are cultivated on cubes of solid medium, surrounded by liquid medium.
This allows changes of media composition at any time. When embryos or callus were produced, the liquid initiation medium was
removed and replaced with regeneration medium. The best yield of embryos and the best regeneration frequency were obtained
when the initiation medium contained 2,4-dichlorophenoxyacetic acid (2,4-D). Regeneration of shoots from callus was stimulated
on double layer medium, with the callus placed on top of a solid medium, partly submerged in the overlaying liquid medium.
The best gelling agent for shoot multiplication media was agarose, but gellan gum was a good alternative. Both the production
of flower buds and embryogenesis in anther cultures was inhibited by an increased concentration of CO2 in the air surrounding the donor plants. |
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| Keywords: | Carbon dioxide gelling agents haploid |
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