Identification of bovine dendritic cell phenotype from bovine peripheral blood |
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| Authors: | Miyazawa Kohtaro Aso Hisashi Honda Masashi Kido Taketomo Minashima Takeshi Kanaya Takashi Watanabe Kouichi Ohwada Shyuichi Rose Michael T Yamaguchi Takahiro |
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| Institution: | Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555 Sendai, Japan. |
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| Abstract: | Dendritic cells (DCs) are professional antigen presenting cells, which initiate primary immune responses and also play an important role in the generation of peripheral tolerance. There is no reliable method established for the isolation of bovine peripheral blood DCs, and furthermore, the phenotypes and the functions of bovine DCs are still not fully clear. In the present study, we have attempted to identify bovine peripheral blood DCs by negative-selection. In bovine peripheral blood mononuclear cells (PBMC), we have newly characterized the phenotype of DCs, which is CD11c+/CD172a+. These cells display features of myeloid type DCs. In the thymic medulla, CD11c+/CD172a+ cells were also present and CD1+/CD172a+ cells were additionally detected as a population of DCs. The data suggest that one of the bovine DCs phenotypes from PBMC is derived from myeloid lineages lacking a CD1 molecule, which then drift to several tissues, and that they then may express a CD1 molecule upon their functional differentiation. |
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| Keywords: | Bovine PBMC Dendritic cells Negative-selection Phenotypes |
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