| 地锦组织培养及无性系建立研究 |
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| 引用本文： | 刘洋,马静,葛宁,徐娜,姜长阳.地锦组织培养及无性系建立研究[J].现代农业科技,2009(21):77-78,81. |
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| 作者姓名： | 刘洋 马静 葛宁 徐娜 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连,116029 |
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| 基金项目： | 辽宁师范大学教学改革项目 |
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| 摘 要： | 以生长旺盛的地锦嫩茎为材料，进行了愈伤组织的诱导和分化、不定芽的分化及试管苗的生根、移栽、扦插和移植的研究。结果证明：MS＋BA0．4～0．6mg／L＋2，4-D1．5～2．0mg／L是诱导嫩茎形成具有分化能力愈伤组织的理想培养基；MS＋AgNO3 0．8mg／L＋BA 0．6mg／L＋NAA 0．1mg／L是诱导愈伤组织分化培养的理想培养基；B5＋BA 0．5mg／L＋NAA 0．1mg／L是不定芽分化培养的理想培养基；B5＋IAA 0．2mg／L＋NAA 0．1mg／L是试管苗生根继代培养的理想培养基；移植的试管苗具有生长旺盛整齐、根系发达、开花结果时间推迟15d左右的特点。
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| 关 键 词： | 地锦 愈伤组织 不定芽 培养基 |
Research on Establishing the Clone of Euphorbia humifusa |
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| Authors: | LIU Yang MA Jing GE Ning XU Na JIANG Chang-yang |
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| Institution: | (College of Life Science, Liaoning Normal University, Dalian Liaoning 116029) |
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| Abstract: | Euphorbia humifusa was used as materials in order to make research on callus induction,adventitious buds differentiation and taking roots,transplanting and cutting of test-tube plantlets.The results indicated that the optimum medium for tender stem inducing and differentiating to callus was MS+BA 0.4~0.6mg/L+2,4-D 1.5~2.0mg/L,the optimum medium for inducing and differentiating callus was MS+ AgNO3 0.8mg/L+BA 0.6mg/L+NAA 0.1mg/L;B5+BA 0.5mg/L+NAA 0.1mg/L was ideal medium for adventitious buds differentiation,B5+IAA 0.2mg/L+NAA 0.1mg/L was ideal medium for adventitious buds taking roots.The florescence could be lengthened 15 days with the vigorous test-tube plantlets and the flourishing roots. |
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| Keywords: | Euphorbia humifusa callus adventitious bud medium |
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