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| 玉米幼胚培养及植株再生的研究 | |
| 引用本文： | 张慧英,张耀华,农友业,薛艳霞.玉米幼胚培养及植株再生的研究[J].玉米科学,2008,16(6):79-82. |
| 作者姓名： | 张慧英 张耀华 农友业 薛艳霞 |
| 作者单位： | 1. 广西大学农学院,南宁,530004 2. 中北大学化工与环境学院;中北大学图书馆,太原,030051 3. 广西大学支农开发中心,南宁,530004 |
| 基金项目： | 广西大学博士启动基金(XO61182) |
| 摘 要： | 以不同玉米自交系幼胚为外植体,研究不同2,4-D浓度对愈伤组织诱导、6-BA浓度对愈伤组织分化和6-BA与NAA配合使用对试管苗生根的影响。结果表明:不同2,4-D浓度对愈伤组织诱导率影响差异不明显,但对胚性率诱导有明显影响,其诱导最佳培养基为MS+2,4-D 2 mg/L+水解酪蛋白500 mg/L+脯氨酸500 mg/L;愈伤组织分化的最佳培养基是MS+6-BA0.5 mg/L+水解酪蛋白500 mg/L+谷氨酰胺200 mg/L+PP333 2 mg/L;试管苗生根的最佳培养基是1/2 MS+6-BA0.5 mg/L+NAA2.0 mg/L+PP333 2.0 mg/L+活性炭0.2%。 |
| 关 键 词： | 玉米 幼胚 愈伤组织 组织培养 |
| 收稿时间： | 2007/12/18 0:00:00 |
| 修稿时间： | 2008/6/27 0:00:00 |
Studied on Tissue Culture and Plant Regeneration of Maize Immature Embryos |
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| ZHANG Hui-ying;ZHANG Yao-hua;XUE Yan-xia.Studied on Tissue Culture and Plant Regeneration of Maize Immature Embryos[J].Journal of Maize Sciences,2008,16(6):79-82. | |
| Authors: | ZHANG Hui-ying;ZHANG Yao-hua;XUE Yan-xia |
| Institution: | ZHANG Hui-ying1,ZHANG Yao-hua2,XUE Yan-xia1(1.College of Agriculture,Guangxi University,Nanning 53004,2.Chemical Industry , Ecology Institute of North University of China,Library of North University of China,Taiyuan 030051,China) |
| Abstract: | The inducement,differentiation and plantlet roots of callus were studied from immature embryos of the different maize inbred lines in vitro on different medium with the different 2,4-D concentration.The results showed that the inducement rate of callus was not significant with the different 2,4-D concentration,but the rate of inducement of EC was significant,and the medium was MS + 2,4-D 2 mg/L + CH 500 mg/L + Pro 500 mg/L.The differentiation of callus was best on the culture medium of 1/2 MS + 6-BA 0.5 mg/L + NAA 2.0 mg/L + PP333 2.0 mg/L+ active carbon MS + 6-BA 0.5 mg/L + CH 500 mg/L + GLU 200 mg/L + PP333 2 mg/L.The plantlet rooting was best on the culture medium of 1/2 MS + 6-BA 0.5 mg/L + NAA 2.0 mg/L + PP333 2.0 mg/L + active carbon 0.2%. |
| Keywords: | Maize Immature embryo Callus Tissue culture |
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