| 串珠镰刀菌孢子表面的一种凝集素 |
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| 引用本文： | 李多川,王恒华,逯静.串珠镰刀菌孢子表面的一种凝集素[J].植物病理学报,2003,33(5):411-414. |
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| 作者姓名： | 李多川 王恒华 逯静 |
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| 作者单位： | 山东农业大学植物病理学系, 泰安 271018 |
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| 基金项目： | the International Science Foundation，国家自然科学基金 |
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| 摘 要： | 用0.2% SDS磷酸缓冲液从串珠镰刀菌(Fusarium moniliforme)孢子表面抽提蛋白质。孢子表面蛋白经DEAE-Sepharose阴离子交换柱层析和Sephacryl S-100分子筛柱层析,分离和纯化了一种凝集素。该凝集素的分子量为70 kDa,糖含量为19.8%,对兔血红细胞有很强的凝集活性,凝集活性被半乳糖特异性地抑制。EDTA和一些金属离子不影响凝集活性。
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| 关 键 词： | 凝集素 串珠镰刀菌 孢子表面 |
A Lectin from Spore Surface of the Plant Pathogenic Fungus Fusarium moniliforme |
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| Abstract.A Lectin from Spore Surface of the Plant Pathogenic Fungus Fusarium moniliforme[J].Acta Phytopathologica Sinica,2003,33(5):411-414. |
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| Authors: | Abstract |
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| Institution: | Department of Plant Pathology, Shandong Agricultural University, Taian 271018, China |
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| Abstract: | A lectin from spore surface of Fusarium moniliforme was purified by DEAE-Sepharose and Sephacryl S-100 chromatography. The purified lectin showed a single band by SDS-PAGE with a molecular weight about 70 kDa. It is a glycoprotein with 19.8 % carbohydrate content. The lectin displayed the ability to markedly agglutinate trypsin-treated rabbit erythrocytes. The agglutination was specifically inhibited by galactose. EDTA and some metal cations showed no effect on lectin activity. |
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| Keywords: | lectin Fusarium moniliforme spore surface |
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