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| 信阳水牛GHRH基因第二外显子的SNPs检测 | |
| 引用本文： | 徐永杰,吴海港,姚瑾,刘英,梁小娟,马云.信阳水牛GHRH基因第二外显子的SNPs检测[J].中国牛业科学,2012,38(4):1-5. |
| 作者姓名： | 徐永杰 吴海港 姚瑾 刘英 梁小娟 马云 |
| 作者单位： | 1. 信阳师范学院生命科学学院,河南信阳,464000 2. 信阳农业高等专科学校,河南信阳,464000 3. 信阳师范学院生命科学学院,河南信阳464000／江苏大学生命科学学院,江苏镇江212013 |
| 基金项目： | 河南省科技攻关项目，河南省高等学校骨干教师资助计划 |
| 摘 要： | 目的]检测信阳水牛群体GHRH基因的SNP多态性。方法]以56头信阳水牛为材料,采集血样,提取基因组DNA,以GenBank公布的牛GHRH基因(gi:194719389)序列作为参照序列,设计PCR引物,采用PCR-SSCP和DNA测序方法对该基因第二外显子的进行突变检测。结果]经SSCP分析发现,这些个体总共出现六种不同的带型。测序结果表明:位于GHRH基因的4 461bp、4 529bp、4 845bp、5 053bp和5 058bp处分别存在C→T、T→G、G→A、G→A和G→T五个单核苷酸突变。结论]表明该基因在信阳水牛群体中单核苷酸多态较为丰富。 |
| 关 键 词： | 信阳水牛 GHRH基因 SNPs PCR-SSCP |
SNPs Detection of GHRH Gene on the 2th Exon Region in Xinyang Buffalo |
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| XU Yong-jie,WU Hai-gang,YAO Jin,LIU Ying,LIANG Xiao-juan,MA Yun.SNPs Detection of GHRH Gene on the 2th Exon Region in Xinyang Buffalo[J].China Cattle Science,2012,38(4):1-5. | |
| Authors: | XU Yong-jie WU Hai-gang YAO Jin LIU Ying LIANG Xiao-juan MA Yun |
| Institution: | 1. College of Life Sciences, Xinyang Normal University, Xinyang, Henan, 464000 2. Xinyang Agricultural College, Xinyang, Henan , 464000 ; 3. College of Life Sciences, J iangsu University, Zhenj iang , J iang su , 212023 ; ) |
| Abstract: | Objective]This study was aimed to detect the SNP of GHRH gene. Method]The Genomic DNA was taken from 56 Xinyang buffalo blood samples. PCR primers were designed according the sequence of GHRH gene （gi. 194719389）. PCR-SSCP and DNA sequencing methods were used to detect the mutations. Results]The results showed that there were six different band types in different individuals by PCR-SSCP analysis. 8 SNPs were detected, located at 4461bp （C→T mutation）,4 529 bp （T→G muta tion）,4 845 bp （G→A mutation）,5 053 bp （G→A mutation）,5 058 bp （G→T mutation）,respectively. Conclusion]The results indicated that GHRH gene was polymorphic in Xinyang buffalo population |
| Keywords: | Xinyang buffalo GHRH gene SNPs PCR-SSCP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |