| 气相色谱法检测牛奶中脂肪酸组分方法的改进 |
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| 引用本文： | 李云龙,杨章平,常玲玲,冀德君,王小龙,甘宗辉,刘姗,刘贤慧.气相色谱法检测牛奶中脂肪酸组分方法的改进[J].中国牛业科学,2012,38(3):18-21. |
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| 作者姓名： | 李云龙 杨章平 常玲玲 冀德君 王小龙 甘宗辉 刘姗 刘贤慧 |
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| 作者单位： | 扬州大学动物科学与技术学院,江苏扬州,225009 |
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| 摘 要： | 目的]为了找到并建立一套简单而高效的方法用来测定牛奶中脂肪酸的组分和含量。方法]应用气相色谱(GC-FID)方法,采用Agilent HP-88专用脂肪酸甲酯毛细管柱和程序升温方法来分离牛奶中的脂肪酸组分,用保留时间定性和峰面积百分比法进行定量。结果]表明:牛奶中的脂肪酸得到了很好的分离,该检测方法的回收率为99.588%,精密度为2.600,具有较高的准确性和重复度。结论]本实验方法可以作为牛奶中脂肪酸检测的技术加以推广和应用。
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| 关 键 词： | 气相色谱 脂肪酸 牛奶 |
The Improvement of Gas Chromatography in Milk Fatty Acid Analysis of Composition |
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| LI Yun-long,YANG Zhang-ping,CHANG Ling-ling,JI De-jun,WANG Xiao-long,GAN Zong-hui,LIU Shan,LIU Xian-hui.The Improvement of Gas Chromatography in Milk Fatty Acid Analysis of Composition[J].China Cattle Science,2012,38(3):18-21. |
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| Authors: | LI Yun-long YANG Zhang-ping CHANG Ling-ling JI De-jun WANG Xiao-long GAN Zong-hui LIU Shan LIU Xian-hui |
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| Institution: | (College of Animal Science and Technology , Yangzhou University , Yangzhou , J iangsu 225009) |
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| Abstract: | Objective]The purpose of this study is to find and establish a simple and efficient method used to detect the fatty acid composition and content in the milk. Method]Gas chromatography （GC-FID） method was used to separate the milk fatty acid composition by using the Agilent HP-88 fatty acid methyl esters capillary column and temperature programmed method, quantitative retention time was used for quantify by qualitative and peak area percentage method. Result]The results showed that the fatty acid in milk was well separated. The recovery and precision of detection method were 99. 588% and 2. 600, respectively, with a high accuracy and repeatability. Result]The experimental method could be used as a milk fatty acid detection technology for promotion and application. |
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| Keywords: | gas chromatography fatty acids milk |
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