Impact of Dietary Cellobiose on the Fecal Microbiota of Horses |
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| Institution: | 1. Racehorse Hospital, Miho Training Center, Japan Racing Association, Ibaraki, Japan;2. Laboratory of Racing Chemistry, Utsunomiya, Tochigi, Japan;3. Racehorse Hospital, Ritto Training Center, Japan Racing Association, Shiga, Japan;4. Equine Department, Japan Racing Association, Tokyo, Japan;1. Medicine and Surgery Department, Copenhagen University, Taastrup, Denmark;2. Universitat Autònoma de Barcelona, Barcelona, Spain;1. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, México;2. Dairy Science Department, National Research Centre, Cairo, Egypt;3. Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Guerrero, Altamirano, Guerrero, México;4. Centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento Animal, INIFAP, Ajuchitlán, Querétaro, México;5. Faculty of Agriculture, Alexandria University, Alexandria, Egypt;1. School of Ecology and Nature Conservation, Beijing Forestry University, Beijing, China;2. Central College, British Columbia, Canada;3. Xinjiang Kalamaili Mountain Ungulate Nature Reserve Management Center, Urumqi, China;4. Xinjiang Research Center for Breeding Przewalski’s Horse, Urumqi, China;1. Department of Clinical Sciences, Ross University, School of Veterinary Medicine, St Kitts;2. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA;3. San Luis Rey Equine Hospital, Bonsall, CA;1. Department of Animal and Dairy Science, University of Georgia, Athens, GA;2. Equine Studies Group, WALTHAM Center for Pet Nutrition, Slough, Berkshire;3. Department of Veterinary Science, University of Kentucky, Lexington, KY;4. Department of Foods and Nutrition, College of Family and Consumer Sciences, University of Georgia, Athens, GA;5. MARS Horsecare US, Dalton, OH;6. Department of Kinesiology, University of Georgia, Athens, GA |
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| Abstract: | Cellobiose is a disaccharide with potential prebiotic effects, as demonstrated in different animal species, but not yet in horses. It was, therefore, the aim of the present study to evaluate the impact of dietary cellobiose on the fecal microbiota of horses. Eight healthy adult horses and two ponies were included in this study. The animals received a diet without or with 10 g and 20 g cellobiose per day for 14 days each. At the end of the feeding periods, fresh fecal samples were collected to measure bacterial metabolites and the microbial composition. For the microbiota analysis, 16S rRNA gene sequencing was used. Cellobiose was well accepted and tolerated by the animals. The lowest fecal concentrations of D-lactate, propionic acid, i-valeric acid, and total short-chain fatty acids were measured at the dose 10 g cellobiose per horse per day (quadratic effect: P < .05). A dose-dependent increase of the relative abundance of Firmicutes (P = .049), Coriobacteriales (P < .001), and Clostridium (P = .031) could be detected. In addition, a dose-dependent decrease of the relative abundance of Bacteroidetes (P = .035) was observed. In conclusion, the increase of Coriobacteriales and Clostridium indicates a bacterial fermentation of cellobiose in the equine intestine, as members of both groups exert saccharolytic activity. As clostridia have previously been assumed to be a key component of the intestinal microbiota in horses, the observed increase of Clostridium in the feces might indicate beneficial and potentially prebiotic effects of cellobiose in horses. However, this finding requires further investigation, particularly with regard to the Clostridium species that have been promoted by dietary cellobiose. |
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| Keywords: | Horses Cellobiose Prebiotic Microbiota Clostridia |
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