A Comparison of Multiple Allergen Simultaneous Tests Using Allergen-Specific IgE Concentration and Intradermal Skin Tests in Atopic Horses With Pollen Allergy |
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| Institution: | 1. Sub-Department of Diagnostics and Veterinary Dermatology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland;2. Institute of Mathematics, Maria Curie-Skłodowska University, Lublin, Poland;3. Equine Breeding and Management Unit, Department of Biology and Animal Breeding,University of Life Sciences in Lublin, Lublin, Poland;4. Nicolaus Copernicus University in Torun, Faculty of Biological and Veterinary Sciences, Torun, Poland;1. Institute of Monitoring of Climatic and Ecological Systems of Siberian Branch of Russian Academy of Science, Akademicheski Ave. 10/3, 634055, Tomsk, Russia;2. Institute of Physicochemical and Biological Problems in Soil Science of Russian Academy of Science, Institutskaya Street 2, Moscow District, 142290, Pushino, Russia;3. The Institute for the History of Material Culture, Russian Academy of Science, Dvortsovaya Nab. 18, 191186, St Petersburg, Russia;4. Department of Geosciences, National Taiwan University, Taipei, 106, Taiwan, ROC;1. Racehorse Hospital, Miho Training Center, Japan Racing Association, Ibaraki, Japan;2. Laboratory of Racing Chemistry, Utsunomiya, Tochigi, Japan;3. Racehorse Hospital, Ritto Training Center, Japan Racing Association, Shiga, Japan;4. Equine Department, Japan Racing Association, Tokyo, Japan;1. Molecular Biology Division, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan;2. Clinical Veterinary Medicine Division, Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan;1. Department of Veterinary Medicine, Animal Reproduction, Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Campo Grande, Brazil;2. Department of Veterinary Medicine, Surgery and Anaesthesiology, Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Campo Grande, Brazil;3. Department of Veterinary Medicine, Federal University of Mato Grosso do Sul, Campo Grande, Brazil;4. Department of Veterinary Medicine, Clinic of Large Animals, Faculty of Veterinary Medicine and Animal Science, Federal University of Mato Grosso do Sul, Campo Grande, Brazil |
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| Abstract: | Intradermal tests (IDTs) and measurement of specific immunoglobulin E class (sIgE) levels in sera are the most common and reliable methods used in allergological clinical practice. The purpose of this study was to explore the sensitization of pollen allergy in atopic horses with pollinosis and to assess the diagnostic value of the multiple allergen simultaneous tests (MASTs) compared with that of the IDT. Twenty-one Malopolski horses with typical skin hypersensitivity symptoms during pollen seasons were enrolled. Intradermal tests were performed, and allergen-specific IgE concentrations in sera were measured using a monoclonal anti-IgE antibody to pollens of grass, weeds, trees, and cultivated plants. The highest sensitization rate was for Secale cereale (IDT, 76%; MAST, 66.7%), grass (IDT, 71%; MAST, 57%), and Brassica napus (IDT, 52.4%; MAST, 57%). Receiver operating characteristic (ROC) curve analysis and the area under the ROC curve (AUC) showed that Betula spp., Alnus spp., and Corylus spp. had the highest AUC at 0.854, followed by Secale cereale (AUC = 0.796), Plantago lanceolata (AUC = 0.726), Brassica napus (AUC = 0.704), and a grass allergen mixture (AUC = 0.695). The mean AUC for all allergens was 0.712 (0.604–0.867). The overall sensitivity of the sIgE plant horse panel was 78% (range, 68%–90%), the specificity was 86.3% (range, 64%–100%), and the accuracy was 79% (range, 64%–87%). Statistical kappa (κ) agreement between the MAST and IDT was reached for tree (κ = 0.767), rye (κ = 0.687), colza (κ = 0.671), and grass (κ = 0.664) pollen. The MAST showed a favorable agreement with the IDT and can be used for the detection of sIgE in atopic horses with pollinosis. |
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| Keywords: | Pollen allergy sIgE Intradermal test ROC curve Horses |
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