Usefulness of a Point-of-Care Analyzer to Measure Cardiac Troponin I and D-Dimer Concentrations in Critically Ill Horses With Gastrointestinal Diseases |
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| Institution: | 1. Animal Medicine Department, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain;2. Veterinary Teaching Hospital, University of Murcia, Murcia, Spain;3. Department of Animal Pathology, Faculty of Veterinary Medicine, University of Murcia, Murcia, Spain;1. Department of Hand and Reconstructive Microsurgery, University Orthopaedics Hand and Reconstructive Microsurgery Cluster, National University Health System, Singapore;2. Department of Orthopaedic Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;1. Department of Pharmacology and Toxicology, Pre-clinical Study Center, Faculty of Medicine, Medical University Sofia, 2, Zdrave str., 1421 Sofia, Bulgaria;2. Department of Internal Medicine, Pharmacology and Clinical Pharmacology, Pediatrics, Epidemiology, Infection Diseases and Dermatology, Faculty of Medicine, Sofia University “St. Kliment Okridsky”, 1, Koziak str., 1407 Sofia, Bulgaria;3. Department of Forensic Medicine, Faculty of Medicine, Medical University Sofia, 2, Zdrave str., 1421 Sofia, Bulgaria;4. Department of Analytical Chemistry, Faculty of Pharmacy, Medical University Sofia, 2, Zdrave str., 1421 Sofia, Bulgaria;5. Department of Forensic Medicine, Military Hospital, 3 “St Georgi Sofiiski str”, 1421 Sofia, Bulgaria;1. Key Laboratory of Cognition and Personality (Southwest University), Ministry of Education, Chongqing, China;2. Faculty of Psychology, Southwest University, Chongqing, China;3. Department of Psychology, University of Nebraska-Lincoln, Lincoln, NE 68588-0308, USA;1. Departamento de Morfologia e Fisiologia Animal, Faculdades de Ciências Agrárias e Veterinárias, UNESP Univ Estadual Paulista, Campus de Jaboticabal, Laboratório de Farmacologia e Fisiologia do Exercício Equino (LAFEQ) Jaboticabal, São Paulo, Brazil;2. Departamento de Tecnologia, Faculdades de Ciências Agrárias e Veterinárias, UNESP Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, São Paulo, Brazil;3. Brazilain Army, Rio de Janeiro, RJ, Brazil;4. Faculdades Metropolitanas Unidas, HOVET, São Paulo, Brazil |
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| Abstract: | Point-of-care (POC) systems for the joint measurement of Troponin and D-dimers have not been studied in horses. The aim of this study was to perform the validation of a POC system (AQT90 FLEX) for the measurement of cardiac troponin I (cTnI) and D-dimers in the serum of horses with gastrointestinal diseases. The main objective was to evaluate whether or not this system can distinguish healthy animals from diseased animals. A sample of 33 horses was included in the study: control group (n = 10) and horses with gastrointestinal disorders (n = 21), which were classified according to their outcome in survivors (subgroup A = 9) and nonsurvivors (subgroup B = 12). Considering the diagnosis of the process, ill horses were classified into three groups: inflammatory (I = 7), obstructive (O = 9), and strangulating diseases (S = 5). The clinical usefulness of AQT90 FLEX was validated by the study of linearity, coefficient of variation, and detection limits. Later, concentrations of D-dimers and cTnI were measured. A significant increase in both parameters was detected in ill animals (cTnI: control: 0.014 ± 0.01 μg/mL, survivors: 0.27 ± 0.37 μg/mL, nonsurvivors: 0.60 ± 1.21 μg/mL; D-dimers: control: 104.90 ± 30.82 ng/mL, survivors: 1,217.22 ± 1,213.28 ng/mL, nonsurvivors: 1,613.67 ± 1,426.75 ng/mL), although there were no statistically significant differences in concentrations according to diagnosis and outcome. In conclusion, AQT90 FLEX POC analyzer can be used in horses with gastrointestinal diseases to measure cTnI and D-dimer concentrations. It is a quick, practical, and minimally invasive tool that helps in determining the severity of illness. |
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| Keywords: | Horse cTnI D-dimers SIRS POC |
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