Does Coenzyme Q10 Exert Antioxidant Effect on Frozen Equine Sperm? |
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| Institution: | 1. Departamento de Clínica e Cirurgia Veterinárias da Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil;2. Departamento de Farmacologia do Instituto de Ciências Biológicas da UFMG, Belo Horizonte, MG, Brazil;3. Faculdade de Medicina Veterinária da Pontifícia Universidade Católica- PUC Minas, Betim, MG, Brazil;1. Dipartimento di Scienze Veterinarie, Università di Pisa, Pisa, Italy;2. Uniwersytet Rolniczy Kraków, Kraków, Poland;3. Practictioner, Grosseto, Italy;1. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan;1. Theriogenology Residency Candidate at Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;2. Department of Animal Reproduction, School of Veterinary Medicine, Shiraz University, Shiraz, Iran;1. Department of Animal Reproduction, Parananense University, Umuarama, Paraná, Brazil;2. Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESP, Botucatu, São Paulo, Brazil;3. Department of Animal Reproduction, Minas Gerais Federal University, Belo Horizonte, Minas Gerais, Brazil;4. Department of Animal Science, Uberaba University, Uberaba, Minas Gerais, Brazil;5. Department of Animal Science, Santo Amaro University, São Paulo, São Paulo, Brazil;1. College of Food Science and Technology, Guangdong Ocean University (Yangjiang Campus), Yangjiang, 529500, China;2. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals, and Guangdong Province Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China;3. Southern Laboratory of Ocean Science and Engineering, Zhuhai, 519000, China;4. Department of Food and Environmental Engineering, Yangjiang Polytechnic, Yangjiang, 529500, China |
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| Abstract: | During semen cryopreservation, the sensitivity of equine sperm to oxidative stress is increased by the eliminated seminal plasma. Thus, antioxidant addition to the semen extender can be helpful to the sperm survival after freezing and thawing. This work aimed to test whether coenzyme Q10 (CoQ10) added in different concentrations to the INRA 82 freezing extender has antioxidant function on equine sperm to improve its fertilizing ability. Semen samples from five stallions were frozen with the extenders: (T1) INRA 82, control, (T2) T1+ 5 μM CoQ10, (T3) T1+ 25 μM CoQ10, and (T4) T1+ 50 μM CoQ10. After sample thawing, sperm motility and kinetics characteristics were evaluated using a computer-assisted sperm analysis and sperm membrane functionality and integrity were evaluated with a hypo-osmotic swelling test and an epifluorescence microscopy, respectively. The nitrite (NO2-) and hydrogen peroxide (H2O2) concentrations of the semen samples were measured with spectrophotometry. There was no difference on the sperm characteristics among all treatments (P > .05). However, the 25 μM CoQ10 (T3) decreased NO2− concentration (6.7 ± 2.2 μM/μg protein) compared with the treatments T1, T2, and T4 (64.3 ± 3.7, 59.4 ± 5.3, 45.1 ± 8.6 μM/μg protein), respectively, as well H2O2 concentration (1.8 ± 0.3 μM/μg protein) compared with the control (4.6 ± 0.4 μM/μg protein) and 5 μM CoQ10 treatments (4.8 ± 0.2 μM/μg protein, P < .05). In conclusion, 25 μM CoQ10 plays a significant role as antioxidant to the frozen equine sperm, decreasing NO2− and H2O2 concentrations. Thus, its addition to the INRA 82 freezing extender may be beneficial to the fertilizing ability of equine semen. |
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| Keywords: | Semen Horse Stallion Spermatozoa Ubiquinone CoQ10 |
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