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Effect of gliadin/glutenin ratio on pasting,thermal, and structural properties of wheat starch
Institution:1. Purdue University Food Science Department, 745 Agriculture Mall Dr West Lafayette, IN 47907;2. Ege University Food Engineering Department, Ege University Campus, Bornova, Izmir, Turkey;3. Izmir Institute of Technology, Department of Chemical Engineering, Urla, Izmir, Turkey;1. Department of Food Science & Technology, College of Agriculture (Nagaur), Agriculture University, Jodhpur, Rajasthan, India;2. Department of Food Science and Technology, Amity University Punjab, Block-D, Sector-82 Alpha, IT City, Sahibzada Ajit Singh Nagar, Punjab, India;3. Department of Food & Nutrition and Food Technology, Institute of Home Economics, University of Delhi, New Delhi, India;4. Department of Food Technology, Guru Jambheshwar University of Science and Technology, Hisar, Haryana, India;5. Indian Council of Agricultural Research-Indian Veterinary Research Institute, Mukteshwar, India;6. Department of Food Science & Technology, Guru Nanak Dev University, Amritsar, Punjab, India
Abstract:Gluten-starch interactions are of specific importance during the processing of cereal-based products. However, the mechanisms for gluten-starch interactions have not been illuminated. The effects of various gliadin/glutenin (gli-glu) ratios (0:10, 3:7, 5:5, 7:3, and 10:0) on the pasting, thermal, and structural properties of wheat gluten-starch mixtures were investigated. The peak, through, and final viscosities were obviously decreased, and the setback value initially increased and then decreased with increasing gli-glu ratios during the rapid viscosity analysis (RVA). Differential scanning calorimetry showed that the enthalpy changes increased with increasing gli-glu ratios. Thermogravimetric analysis showed a slight increase in the degradation temperature of the mixtures as the gli-glu ratio increased, although it was still lower than that of wheat flour. However, there was no significant difference in the weight loss among different gli-glu ratios. Rheometer-Fourier transform infrared (FTIR) spectroscopy showed that the C-6 peak at 996 cm−1 for all the samples was displaced or disappeared due to the hydrogen bond fracture caused by water molecules entering the starch granules. It was also found that the absorption peak in amide II of gli-starch was more obvious than that of glu-starch. The CLSM obviously described the change structure of mixtures with different gli-glu ratio during starch gelatinizaton. By studying the changes in gluten protein components and how they affected the thermal and structural properties of starch, a simple model was proposed to describe the gelatinization process of the mixtures with different ratios of gli-glu and briefly describe the interactions between starch and wheat gluten components. Optimization of the proportion of protein components in wheat flour will enable greater control over the structural characteristics and elasticity of wheat food products.
Keywords:Gliadin-glutenin  Starch  Thermal  Rheometer-FTIR  Model
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