| 鸡白痢和鸡伤寒沙门氏菌的PCR-RFLP分子鉴别 |
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| 引用本文： | 徐耀辉,焦新安,胡青海,焦凤超,曾显营,潘志明,黄金林.鸡白痢和鸡伤寒沙门氏菌的PCR-RFLP分子鉴别[J].扬州大学学报(农业与生命科学版),2005,26(1):1-4,29. |
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| 作者姓名： | 徐耀辉 焦新安 胡青海 焦凤超 曾显营 潘志明 黄金林 |
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| 作者单位： | 扬州大学,生物科学与技术学院;扬州大学,兽医学院,江苏,扬州,225009;扬州大学,生物科学与技术学院 |
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| 基金项目： | 国家自然科学基金资助项目(30425031)
国家高技术发展计划项目(863-2003AA222141) |
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| 摘 要： | 根据鸡白痢沙门氏菌和鸡伤寒沙门氏菌fliC基因可变区两端的保守序列设计1对引物，PCR扩增出约600bp的产物，用Hin6I对PCR产物进行酶切，经RFLP分析区分鸡白痢沙门氏菌、鸡伤寒沙门氏菌的生物型。利用该技术对6株鸡白痢沙门氏菌标准株及2株鸡伤寒沙门氏菌标准株进行分子鉴别，得到的结果与预计的RFLP模式相符，证明该方法可行。在此基础上对我国不同地区、不同年代的191株鸡沙门氏菌进行分子鉴别，191株中有185株分离株符合鸡白痢沙门氏菌的RFLP模式，6株分离株符合鸡伤寒沙门氏菌的RFLP模式，其中有176株PCR—RFLP鉴定结果与生化特性符合率达100％，另有15株根据生化特性不能鉴别。
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| 关 键 词： | 鸡白痢沙门氏菌 鸡伤寒沙门氏菌 PCR-RFLP fliC基因 分子鉴别 |
| 文章编号： | 1671-4652(2005)01-0001-04 |
Molecular differentiation of Salmonella pullorum from Salmonella gallinarum by PCR-RFLP |
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| XU Yao-hui,JIAO Xin-an,HU Qing-hai,JIAO Feng-chao,ZENG Xian-ying,PAN Zhi-ming,HUANG Jin-lin.Molecular differentiation of Salmonella pullorum from Salmonella gallinarum by PCR-RFLP[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2005,26(1):1-4,29. |
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| Authors: | XU Yao-hui JIAO Xin-an HU Qing-hai JIAO Feng-chao ZENG Xian-ying PAN Zhi-ming HUANG Jin-lin |
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| Abstract: | According to the conserved sequence of the variable region of fliC gene from S. pullorum and S. gallinarum, a pair of specific primers were designed. The PCR amplified product, 600 bp fragment was digested with Hin6 I . S. pullorum and S. gallinarum were able to be differentiated by PCR-RFLP. By using this method, the 6 reference strains of S. pullorum and 2 reference strains of S. gallinarum were identified, with the results matching the predicted patterns. It could be concluded that this method is feasible. Furthermore, 191 isolates were differentiated, 185 of them were S. pullorum, while other 6 isolates were identified as S. gallinarum. The typing results of 176 isolates were identical between biochemical typing and PCR-RFLP, but 15 isolates were unable to be typed by biochemical traits. |
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| Keywords: | Salmonella pullorum Salmonella gallinarum PCR-RFLP fliC gene molecular differentiation |
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