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| 玉米品种DNA指纹数据库构建的标准化规范 | |
| 引用本文： | 王凤格,赵久然,戴景瑞,郭景伦,原亚萍,王璐,易红梅,孙世贤,吕波.玉米品种DNA指纹数据库构建的标准化规范[J].分子植物育种,2007,5(1):128-132. |
| 作者姓名： | 王凤格 赵久然 戴景瑞 郭景伦 原亚萍 王璐 易红梅 孙世贤 吕波 |
| 作者单位： | 1. 中国农业大学农学与生物技术学院,北京,100094;北京市农林科学院玉米研究中心,北京,100097 2. 北京市农林科学院玉米研究中心,北京,100097 3. 中国农业大学农学与生物技术学院,北京,100094 4. 吉林大学植物科学学院,长春,130062 5. 全国农技推广服务中心,北京,100026 6. 农业部品种测试中心,北京,100026 |
| 基金项目： | 北京市农业育种基础研究创新平台项目 , 北京市自然科学基金 , 北京市科技新星计划项目 , 吉林省科技基金 |
| 摘 要： | 为了保证玉米品种DNA指纹库构建的标准化,从建库标记、检测平台、试剂、样品、评估程序、数据整合、模式库、扩展库、随机盲测等九个方面进行了全面的规范:(1)通过对不同分子标记的比较,确定SSR标记作为建库标记;(2)通过对不同DNA指纹检测方法的比较,确定毛细管电泳结合多色荧光检测方法作为DNA指纹片段分析方法;(3)规范了DNA、引物、Taq酶等试剂质量,以保证数据的稳定性和可重复性;(4)规范了样品来源、样品类型及分析样品数量;(5)确定评估品种的数量及引物的取舍标准;(6)为解决不同来源DNA指纹数据的有效整合问题,提出了固定一套核心引物、提供标准品种、提供标准DNA、确定引物等位基因BIN、规定对异常带型数据处理方式、规定数据的编码方式等6个解决策略;(7)规范了构建模式库的材料名单、来源及构建方式;(8)规范了构建扩展库的材料来源及构建方式;(9)进行数据库数据随机盲测,以评估入库数据质量.以上规范将为不同单位合作开展大规模的DNA指纹库构建研究奠定基础. |
| 关 键 词： | 玉米 DNA指纹 数据库 |
Criteria for the Construction of Maize DNA Fingerprint Database |
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| Wang Fengge,Zhao Jiuran,Dai Jingrui,Guo Jinglun,Yuan Yaping,Wang Lu,Yi Hongmei,Sun Shixian,Lv Bo.Criteria for the Construction of Maize DNA Fingerprint Database[J].Molecular Plant Breeding,2007,5(1):128-132. | |
| Authors: | Wang Fengge Zhao Jiuran Dai Jingrui Guo Jinglun Yuan Yaping Wang Lu Yi Hongmei Sun Shixian Lv Bo |
| Abstract: | In order to standardize the construction of Chinese maize DNA fi ngerprint database, a comprehensive criterion was set up, which regulated nine related aspects including molecular marker, detection platform, reagent, sample, evaluation procedure, data conformity, pattern database, extended database, blind test: (1) select SSR marker for the database construction after comparing different molecular markers; (2) select the fi ve-color ? uorescence capillary detection system for DNA fi ngerprint analysis after comparing different detection systems; (3) in order to guarantee the stability and repeatability of data, standardize the reagent quality of DNA, primer, Taq et al.; (4) standardize sample source, type and quantity; (5) determine quantity of varieties for evaluation and selection criteria of primers; (6) in order to assure conformity of DNA fi ngerprint data from different source, six schemes were suggested including fi xing a set of core primes, offering standard varieties, offering standard DNA, determining BIN of primers, regulating how to deal with abnormal band types and setting down way of data coding; (7) standardize variety list, source and method for constructing the pattern database; (8) standardize variety source and method for constructing the extended database; (9) carry out blind test to the data in DNA database.These criterions would be helpful for collaboration among different laboratories to construct high-throughput DNA fi ngerprint database. |
| Keywords: | Maize DNA fingerprint Database |
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