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重金属胁迫培养对2种细菌生长曲线的影响(摘要)(英文)
引用本文:李淑英,苏亚丽,周元清,史云东,别金蓉.重金属胁迫培养对2种细菌生长曲线的影响(摘要)(英文)[J].农业科学与技术,2010(8):149-152.
作者姓名:李淑英  苏亚丽  周元清  史云东  别金蓉
作者单位:玉溪师范学院资源环境学院,云南玉溪653100
基金项目:云南省教育厅科学研究基金项目“重金属对几种微生物的胁迫生理毒性研究”(09Y0382);云南省自然基金项目“重金属污染中三种水生植物的毒害机理研究”(2008ZC161M).
摘    要:目的]研究重金属胁迫培养对2种细菌生长曲线的影响。方法]以大肠杆菌和枯草芽孢杆菌2种典型细菌为试验材料,采用传统培养方法,选择5种重金属离子Cu2+、Hg2+、Pb2+、Cd2+、Cr6+在不同浓度下对其进行胁迫培养,通过测定2种细菌的生长曲线,研究外源性重金属对2种细菌生长的影响。结果]Hg2+、Cd2+毒性较强,2种细菌的增殖在高浓度时受到抑制,G+比G-更为敏感;当重金属浓度50mg/L后,5种重金属的对2种细菌的毒性顺序为Hg2+Cd2+Cu2+Cr6+≈Pb2+。结论]该研究结果为进一步研究重金属对环境及生态系统的影响奠定基础。

关 键 词:重金属  大肠杆菌  枯草芽孢杆菌  生长曲线  胁迫

Effects of Heavy Metal Stress on the Growth Curves of Two Sepcies of Bacteria
LI Shu-ying,SU Ya-li,ZHOU Yuan-qing,SHI Yun-dong,BIE Jin-rong.Effects of Heavy Metal Stress on the Growth Curves of Two Sepcies of Bacteria[J].Agricultural Science & Technology,2010(8):149-152.
Authors:LI Shu-ying  SU Ya-li  ZHOU Yuan-qing  SHI Yun-dong  BIE Jin-rong
Institution:College of Resources and Environment,Yuxi Normal University,Yuxi 653100
Abstract:Objective] The aim was to study on the effects of heavy metal stress on the growth curves of Escherichia coli and Bacillus subtili. Method] Using traditional culture method,Escherichia coli and Bacillus subtili were cultured under heavy metal stress including Cu2+,Hg2+,Pb2+,Cd2+ and Cr6+ in different concentrations. Then the growth curves of the bacteria were determined to investigate the effects of exogenous heavy metals on the growth of the two kinds of bacteria. Result] The proliferation of the two bacteria was inhibited at high concentrations of Hg2+ and Cd2+ respectively,and G+ is more sensitive to them than G-; when the heavy metal concentration was 50 mg/L,the toxicity of the five kinds of heavy metals on the two bacteria was Hg2+Cd2+Cu2+Cr6+≈Pb2+. Conclusion] The research will provide a basis to explore the effects of heavy metal on environment and ecological system.
Keywords:Heavy metal  Escherichia coli  Bacillus subtili  Growth curve  Stress
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