| 产胞外淀粉酶枯草芽孢杆菌的分离筛选及其紫外诱变育种 |
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| 引用本文： | 谢凤行,赵玉洁,周可,张峰峰,李亚玲.产胞外淀粉酶枯草芽孢杆菌的分离筛选及其紫外诱变育种[J].华北农学报,2009,24(3). |
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| 作者姓名： | 谢凤行 赵玉洁 周可 张峰峰 李亚玲 |
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| 作者单位： | 天津市农业生物技术研究中心,天津,300192 |
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| 基金项目： | 天津市应用基础研究计划项目，天津市农业科学院院长基金项目 |
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| 摘 要： | 为提高枯草芽孢杆菌产生淀粉酶能力,从养虾池、混养池及污染河流的底质活性污泥中分离到12株枯草芽孢杆菌,通过淀粉降解试验筛选到产酶能力较高的菌株H4和H5,菌株淀粉酶活性分别为38.66,37.10 U/mL.以筛选到H4和H5为原始菌株,采用紫外诱变的方法对H4和H5进行连续诱变筛选产淀粉酶高的突变株.结果发现,第一次诱变后突变株的酶活分别为原始菌株的107%和111%;经过第二次诱变后,H4的突变株H4 Ⅱa,H5的突变株H5Ⅱa和H5Ⅱb的产酶能力有很大程度的提高,分别为原始菌株的147%,136%和135%,酶活达56.95,50.47和50.02 U/mL,说明紫外连续诱变有利于突变株产酶能力的提高.
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| 关 键 词： | 胞外淀粉酶 枯草芽孢杆菌 分离 筛选 紫外诱变 |
The Isolation,Screening and Ultraviolet Mutation Breeding of Bacillus subtilis Strains Producing Extracellular Amylase |
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| XIE Feng-xing,ZHAO Yu-jie,ZHOU Ke,ZHANG Feng-feng,LI Ya-ling.The Isolation,Screening and Ultraviolet Mutation Breeding of Bacillus subtilis Strains Producing Extracellular Amylase[J].Acta Agriculturae Boreali-Sinica,2009,24(3). |
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| Authors: | XIE Feng-xing ZHAO Yu-jie ZHOU Ke ZHANG Feng-feng LI Ya-ling |
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| Institution: | Tianjin Research Center of Agricultural Biotechnology;Tianjin 300192;China |
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| Abstract: | Bacillus subtilis used as probiotic was widely applied to the aquiculture to purify the water with the needs of healthy breeding.In this paper twelve Bacillus subtilis strains were isolated from activate sludge of breeding shrimp pond,breeding fish pond and polluted river.H4 and H5 with high amylase activity were screened from the isolated strains by amylum degradation experiment and the activities of amylase were 38.66,37.10 U/mL,respectively.The mean of ultraviolet continuous induced mutation was used on ... |
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| Keywords: | Extracellular amylase Bacillus subtilis Isolation Screening Ultraviolet mutation |
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