| 乙脑病毒CN株PrM/E基因的克隆及序列分析 |
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| 引用本文： | 汤德元,郭万柱,徐志文,颜其贵,王印,王晓玉.乙脑病毒CN株PrM/E基因的克隆及序列分析[J].四川农业大学学报,2004,22(3):252-256. |
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| 作者姓名： | 汤德元 郭万柱 徐志文 颜其贵 王印 王晓玉 |
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| 作者单位： | 1. 四川农业大学,动物科技学院,四川,雅安,625014;贵州大学,动物科学学院,贵州,贵阳,550025
2. 四川农业大学,动物科技学院,四川,雅安,625014 |
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| 摘 要： | 通过RT -PCR扩增乙型脑炎病毒CN株主要抗原基因PrM /E ,并将PrM /E基因克隆、测序后 ,与GenbanK发表的JEVSA14 (U14 16 3)基因序列进行比较分析 ,发现CN株与JEVSA14强毒株的同源性为 98%。在JEV基因组 5′端 4 77～ 2 4 77的长 2 0 0 0nt的决定JEV抗原性的PrM /E区中 ,CN株与SA14株有 4 0个碱基不同 ,其中 2 8个碱基突变为同义突变 ,另外 12个为错义突变 ,导致相应的氨基酸序列 (6 6 6个 )中的 4个氨基酸发生了突变 ,其中有 2个氨基酸出现在含有关键的抗原决定簇的E蛋白内 ,但不影响其功能表达
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| 关 键 词： | 日本乙型脑炎病毒 PrM/E基因克隆 核酸序列 |
| 文章编号： | 1000-2650(2004)03-0252-05 |
| 修稿时间： | 2004年5月20日 |
Cloning and Sequencing of PrM/E Genes of Japanese Encephalitis Virus (JEV) CN Strain |
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| TANG De-yuan,GUO Wan-zhu,XU Zhi-wen,Yan Qi-gui,Wang Yin,Wang Xiao-yu.Cloning and Sequencing of PrM/E Genes of Japanese Encephalitis Virus (JEV) CN Strain[J].Journal of Sichuan Agricultural University,2004,22(3):252-256. |
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| Authors: | TANG De-yuan GUO Wan-zhu XU Zhi-wen Yan Qi-gui Wang Yin Wang Xiao-yu |
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| Institution: | TANG De-yuan~1,2,GUO Wan-zhu~1,XU Zhi-wen~1,Yan Qi-gui~1,Wang Yin~1,Wang Xiao-yu~1 |
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| Abstract: | This paper reports the cloning of the PrM/E gene of the Japanese Enceplialitis Virus (JEV) CN strain by RT-PCR and analysis of the gene sequence. Comparison of the gene sequence with that of the JEV SA14 strain demonstrates 98% sequence homology. In the 2?Kb genome region of the virus, between nucleotides 477～2477, 40 base pairs differe between the two strain. Only 12 of these differences are missense mutations that result in 4 amino acid differences in the protein sequences. Two of the amino acid substitutions occur in the region encoding for the E protein and do not cause a change in the activity of this protein between the two strains of JEV. |
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| Keywords: | Japanese encephalitis virus PrM/E genes cloning sequence analysis |
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