| HaNPV病毒对寄主昆虫的持续作用研究 |
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| 引用本文： | 曲良建,张永安,王玉珠,郎杏茹.HaNPV病毒对寄主昆虫的持续作用研究[J].林业科学研究,2006,19(2):216-220. |
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| 作者姓名： | 曲良建 张永安 王玉珠 郎杏茹 |
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| 作者单位： | 1. 中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室,北京,100091
2. 宁夏森林病虫害防治检疫总站,宁夏,银川,750001 |
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| 摘 要： | 用不同浓度的HaNPV病毒感染3龄棉铃虫幼虫并分别收集不同处理的存活试虫进行室内传代饲养.结果表明,病毒对寄主昆虫不仅具有直接致死作用,而且对蛹质量和化蛹均有明显的影响,尤其是对亲代和子一代的寄主昆虫.同时,通过PCR技术,从子一代的幼虫和蛹中成功检测到该病毒,从而在分子水平上证明了病毒能够经过亲代传递到子代的种群中.这对病毒的高效利用和害虫的持续控制具有重要的理论和实践意义.
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| 关 键 词： | 持续控制 棉铃虫核型多角体病毒 |
| 文章编号： | 1001-1498（2006）02-0216-05 |
| 收稿时间： | 2005-11-09 |
| 修稿时间： | 2005-11-09 |
Studies on Sustainable Effects for Helicoverpa armigera Nuclear Polyhedrosis Virus to Host Insects |
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| QU Liang-jian,ZHANG Yong-an,WANG Yu-zhu and LANG Xing-ru.Studies on Sustainable Effects for Helicoverpa armigera Nuclear Polyhedrosis Virus to Host Insects[J].Forest Research,2006,19(2):216-220. |
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| Authors: | QU Liang-jian ZHANG Yong-an WANG Yu-zhu and LANG Xing-ru |
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| Institution: | 1. Research Institute of Forest Ecology, Environment and Protection, CAF, Key Laboratory of Forestry Protection,State Forestry Administration , Beijing 100091, China;2. Center Station of Control and Quarantine of Forest Diseases and Pests , Yinchuan 750001, Ningxia, China |
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| Abstract: | Third instars larvae of cotton bollworm,Helicoverpa armigera,were infected with different concentrations of Helicoverpa armigera nucleopolyhedrovirus(HaNPV).Survivors were collected respectively and reared in laboratory room.Mortality rate of the larvae,average weight of the single pupa and pupation rate were observed in parental generation and successive filial generations.The results showed that the virus not only led to death of larvae in parental and folial generation,but also impacted the average weight of the single pupa and pupation rate,especially to the parental and the first folial generation.The experimental data indicated that the virus could weaken the host in folial generations,which could control populations of the host for a long time.Meanwhile,polymerase chain reaction(PCR) amplification of polyhedrin gene sequences demonstrated that the virus was present in eggs and pupae whose parents were infected.This method might provide a better understanding of nucleopolyhedrovirus which could transmit from parental generation to folial generation. The research would play an important role in utilizing the virus effectively to control the pest persistently. |
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| Keywords: | PCR |
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