| PYY对LPS诱导小鼠腹腔巨噬细胞TNF-α、IL-6分泌的影响 |
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| 引用本文： | 李志强,;崔敏,;陈福军,;李苏楠,;张晓静,;薛文静,;刘红梅,;陈巍,;柳巨雄.PYY对LPS诱导小鼠腹腔巨噬细胞TNF-α、IL-6分泌的影响[J].兽医大学学报,2014(4):588-592. |
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| 作者姓名： | 李志强 ;崔敏 ;陈福军 ;李苏楠 ;张晓静 ;薛文静 ;刘红梅 ;陈巍 ;柳巨雄 |
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| 作者单位： | [1]吉林大学动物医学学院,吉林长春130062; [2]长春五棵树经济开发区动物卫生监督所,吉林长春130401 |
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| 基金项目： | 吉林大学种子基金资助项目（450060481363）; 吉林大学农学部青年科研基金资助项目（4305050102P4）; 吉林大学《大学生创新创业训练计划资助》项目（2012C81365） |
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| 摘 要： | 为明确PYY对巨噬细胞炎性细胞因子分泌的调节作用,本试验分离培养健康小鼠腹腔巨噬细胞,不同浓度PYY预处理后,以LPS刺激。ELISA方法检测细胞培养上清中TNF-α、IL-6含量,半定量PCR方法检测细胞中TNF-α、IL-6mRNA表达变化。结果显示：高浓度的PYY1-36（10-9-10-7 mol/L）和PYY3-36（10-8-10-7 mol/L）对LPS诱导小鼠腹腔巨噬细胞TNF-α分泌具有显著抑制作用（P〈0.05）;PYY1-36对LPS诱导小鼠腹腔巨噬细胞IL-6分泌无明显作用（P〉0.05）;不同浓度PYY3-36（10-11-10-7 mol/L）对LPS诱导小鼠腹腔巨噬细胞IL-6分泌均具有显著抑制作用（P〈0.05）。表明PYY对LPS诱导小鼠腹腔巨噬细胞炎性细胞因子TNF-α及IL-6的分泌具有一定的抑制作用,提示PYY可能通过抑制炎性细胞因子的分泌而抑制炎症性疾病的发生发展。
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| 关 键 词： | PYY 巨噬细胞 炎性细胞因子 小鼠 |
Effects of peptide YY on secretion of TNF-a and IL-6 in LPS-induced mouse peri- toneal macrophage |
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| Institution: | LI Zhi-qiang ,CUI Min ,CHEN Fu-jun , LI Su-nan , ZHANG Xiao-jing, XUE Wen-jing , LIU Hong- mei , CHEN Wei , LIU Ju-xiong ( 1. College of Veterinary Medicine, Jilin University, Chang- chun 130062 ,China ;2. Animal Health Inspection Institute of Wukeshu Economic Development zone in Changchun , Changchun 130401, China) |
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| Abstract: | To investigate the effects of peptide YY（PYY）on secretion of pro-inflammatory cyto- kines in maerophage,isolated mouse peritoneal macrophages that had been pretreated with peptide YY at different concentration were stimulated with LPS. The levels of tumor necrosis factor-a （TNF-α） and interleukin-6 （IL-6） in culture supernatant were determined with enzyme-linked im- munosorbent assay. The mRNA expression of TNF-α and IL-6 were detected with PCR. The result showed that the LPS-induced TNF-α production in mouse peritoneal macrophage was suppressed by higher concentration of PYY1-36（10^-8-10^-7 mol/L） or PYY3-36（10^-8-10^-7 mol/L） （P〈0.05）; the LPS-indueed IL-6 production in mouse peritoneal macrophage was suppressed by PYY3-36 at wide rangeof concentration（10^-8-10^-7 mol/L） （P〈0.05）,but not PYY1-36 （P〉0.05）. Our results provide evidence that PYY inhibits to some degree the secretion of pro-inflam- matory cytokines TNF-α and IL 6 in mouse peritoneal macrophage. These data suggest that PYY may inhibit occurrence and development of inflammatory diseases by suppressing secretion of pro- inflammatory cytokines. |
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| Keywords: | PYY macrophage pro-inflammatory cytokines mouse |
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