| 农杆菌不同菌液浓度对柳枝稷种子萌发及幼苗生长发育的影响 |
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| 引用本文： | 徐开杰,燕明献,柴乖强,孙风丽,刘曙东,奚亚军.农杆菌不同菌液浓度对柳枝稷种子萌发及幼苗生长发育的影响[J].草业学报,2012,21(1):206-212. |
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| 作者姓名： | 徐开杰 燕明献 柴乖强 孙风丽 刘曙东 奚亚军 |
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| 作者单位： | 西北农林科技大学农学院,陕西杨凌,712100;西北农林科技大学农学院,陕西杨凌,712100;西北农林科技大学农学院,陕西杨凌,712100;西北农林科技大学农学院,陕西杨凌,712100;西北农林科技大学农学院,陕西杨凌,712100;西北农林科技大学农学院,陕西杨凌,712100 |
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| 基金项目： | 国家自然科学基金，国家948计划项目，西北农林科技大学基本科研业务费专项资金 |
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| 摘 要： | 以柳枝稷品系‘西稷１号’、‘西稷２号’和‘西稷３号’为主区，以农杆菌菌液浓度为副区，对农杆菌浸种处理后柳枝稷种子萌发及幼苗生长发育相关性状进行了研究。结果表明，品系与农杆菌菌液浓度的互作效应不显著；不同基因型品系对农杆菌的敏感程度不同，其中西稷３号最不敏感；随着农杆菌菌液浓度的升高，柳枝稷种子发芽率、幼苗成苗率及叶绿素含量呈下降趋势，幼苗ＰＯＤ 活性、ＣＡＴ 活性、ＭＤＡ 含量呈上升趋势，ＳＯＤ 活性呈先升后降的趋势。综合农杆菌对柳枝稷种子萌发及幼苗生理生化特性影响，在柳枝稷农杆菌浸种转化法中菌液浓度控制在１．２ＯＤ６００较为适宜。
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| 关 键 词： | 柳枝稷 农杆菌 菌液浓度 浸种 |
Switchgrass seeds germination and seedling growth and development with different concentration of Agrobacterium tume f aciens |
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| XU Kai-jie
,
YAN Ming-xian
,
CHAI Guai-qiang
,
SUN Feng-li
,
LIU Shu-dong
,
XI Ya-jun.Switchgrass seeds germination and seedling growth and development with different concentration of Agrobacterium tume f aciens[J].Acta Prataculturae Sinica,2012,21(1):206-212. |
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| Authors: | XU Kai-jie YAN Ming-xian CHAI Guai-qiang SUN Feng-li LIU Shu-dong XI Ya-jun |
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| Institution: | (College of Agriculture,Northwest Agriculture and Forestry University,Yangling 712100,China) |
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| Abstract: | With the switchgrass strains(Xiji No.1,Xiji No.2,Xiji No.3) as the dominated factors,the concentration of Agrobacterium tumefaciens as the deputy factors,the effect of switchgrass seed germination and seedling physio-biochemical characteristics were studied,using A.tumefaciens-soaking method.The results showed that the interaction effect between the strains and the concentration was not significant;The effects of A.tumefaciens on switchgrass strains were different,and the Xiji No.3 was more insensitive to A.tumefaciens than the other strains.With the concentration of A.tumefaciens increased,the switchgrass seed germination rate,the seedling plantingpercent and chlorophyll content showed downtrend,and the seedling POD activity,CAT activity and MDA content showed uptrend,and the seedling SOD activity initially increased but then decreased.To sum up,the most appropiate concentration of A.tumefaciens may be under 1.2 OD600 in switchgrass seeds soaking. |
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| Keywords: | switchgrass(Panicum virgatum) Agrobacterium tumefaciens concentration soaking seed |
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