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水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响
引用本文:王玉萍,董雯,张鑫,杨茜,张峰.水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响[J].草业学报,2012,21(1):213-219.
作者姓名:王玉萍  董雯  张鑫  杨茜  张峰
作者单位:1.甘肃农业大学农学院 甘肃省干旱生境作物学重点实验室 甘肃省作物遗传改良与种质创新重点实验室,甘肃 兰州 730070;2.甘肃农业大学生命科学技术学院,甘肃 兰州 730070
基金项目:甘肃省自然科学基金,国家自然科学基金
摘    要:以先华70和祁连白雪2个耐盐性不同的花椰菜品种为材料,在125 mmol/L NaCl胁迫处理下,研究了不同浓度水杨酸对花椰菜种子萌发及幼苗生理特性的影响。结果表明,盐胁迫下,0.5~1.5 mmol/L SA浸种预处理的花椰菜种子具有较高的发芽率、发芽势、发芽指数及ɑ-淀粉酶活性,其中以1.0 mmol/L的SA处理效果最好;0.5~1.5 mmol/L SA处理的花椰菜幼苗具有较高的株高、茎粗和较大的叶面积,其叶绿素含量、可溶性糖含量、脯氨酸含量以及超氧化物歧化酶(SOD)和过氧化物酶(POD)的活性均较高,而丙二醛(MDA)含量较低,其中以1.0 mmol/L SA处理效果最好。说明0.5~1.5 mmol/L外源SA可以通过提高ɑ-淀粉酶活性,提高花椰菜幼苗渗透调节能力和抗氧化能力,诱导花椰菜种子萌发和幼苗的耐盐性,减轻和缓解盐害,SA浓度超过2.0 mmol/L时对盐胁迫没有缓解效应。

关 键 词:花椰菜  水杨酸  种子萌发  幼苗  NaCl胁迫

Effects of salicylic acid on seed germination and physiological characters of cauliflower seedlings under salt stress
WANG Yu-ping , DONG Wen , ZHANG Xin , YANG Qian , ZHANG Feng.Effects of salicylic acid on seed germination and physiological characters of cauliflower seedlings under salt stress[J].Acta Prataculturae Sinica,2012,21(1):213-219.
Authors:WANG Yu-ping  DONG Wen  ZHANG Xin  YANG Qian  ZHANG Feng
Institution:1.College of Agronomy, Gansu Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Genetic & Germplasm Enhancement, Gansu Agricultural University, Lanzhou 730070, China; 2.College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
Abstract:Effects of salicylic acid on salt stress-induced injuries were investigated during the stage of seed germination and seedling growth of cauliflower(Brassica oleracea var.botrytis).Under the stress,presoaking the seeds with 0.5-1.5 mmol/L of salicylic acid increased the germination percentage,germination energy,germination index and relative α-amylase activity of cauliflower.Among the treatment of salicylic acid,1.0 mmol/L had the best effect.The results also showed that treatment with 0.5-1.5 mmol/L of salicylic acid during the stage of seedling growth under salt stress increased the plant height,stem diameter,leaf area,chlorophyll contents,free proline and water dissolved-carbohydrate contents,the activities of superoxide dismutase(SOD) and peroxidase(POD),and decreased the content of malondialdehyde(MDA),suggesting that the exogenous SA(salicylic acid) could effectively mitigate the injuries of salt stress and increase salt-tolerance of cauliflower seeds and seedlings by increasing α-amylase activity,osmotic regulation and antioxidation.The best treatment concentration of salicylic acid was 1.0 mmol/L.When the concentration of SA was beyond 2.0 mmol/L,no mitigation effect was observed on the seeds germination and growth of seedling under salt stress.
Keywords:cauliflower(Brassica oleracea var  botrytis)  salicylic acid  seed germination  seedling growth  salt stress
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