Comparative susceptibility of embryonated hen eggs and pig lung alveolar macrophages to chlamydial infection |
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| Authors: | J W C Hood D A McMartin |
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| Institution: | (1) Veterinary Laboratory, Ministry of Agriculture, Fisheries and Food, Eskgrove, EH18 1HU Lasswade, Midlothian, UK;(2) Veterinary Extension, University of California, 405 Surge IV Complex, Davis, California, (U.S.A.) |
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| Abstract: | Pig lung alveolar macrophages provided a suitable cell culture system for primary isolation of ovine chlamydia and sustained their growth through several passages. In centrifuged preparations, titres of established strains compared favourably with those obtained in fertile eggs, and in parallel titrations on primary isolation, evidence of chlamydial infection was detectable in macrophages several days earlier than in eggs. |
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