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海藻肥对低温胁迫下铁皮石斛抗氧化能力及相关基因表达的影响
引用本文:王旭承,王婷,王梦娇,李振锡,崔永一.海藻肥对低温胁迫下铁皮石斛抗氧化能力及相关基因表达的影响[J].中国土壤与肥料,2022(1):194-201.
作者姓名:王旭承  王婷  王梦娇  李振锡  崔永一
作者单位:浙江农林大学农业与食品科学学院;杭州青洋科技有限公司
基金项目:浙江省科技援助项目(2020C26003)。
摘    要:以铁皮石斛'晶品一号'幼苗为试材,分别于叶面喷施不同稀释倍数(500、1000、1500倍)的海藻肥,研究其对4℃低温胁迫下铁皮石斛抗氧化生理特性及相关基因表达的影响.结果 表明:1000倍稀释浓度的海藻肥有效缓解了低温胁迫下铁皮石斛叶片失活、叶绿素降解和丙二醛(MDA)积累;促进了脯氨酸(Pro)含量的增加,以及抗氧...

关 键 词:海藻肥  铁皮石斛’晶品一号’  低温胁迫  抗氧化生理  基因表达
收稿时间:2020/9/22 0:00:00

Effects of seaweed fertilizer on antioxidant capacity and related gene expression of Dendrobium officinale under low-temperature stress
WANG Xu-cheng,WANG Ting,WANG Meng-jiao,LI Zhen-xi,CUI Yong-yi.Effects of seaweed fertilizer on antioxidant capacity and related gene expression of Dendrobium officinale under low-temperature stress[J].Soil and Fertilizer Sciences in China,2022(1):194-201.
Authors:WANG Xu-cheng  WANG Ting  WANG Meng-jiao  LI Zhen-xi  CUI Yong-yi
Institution:(The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province,College of Agriculture and Food Sciences,Zhejiang Agriculture and Forestry University,Hangzhou Zhejiang 311300;Hangzhou Qingyang Technology Co.,Ltd.,Hangzhou Zhejiang 311100)
Abstract:To explore the effects of different concentrations of seaweed fertilizer on the antioxidant physiology characteristics and related gene expression level of Dendrobium officinale under low-temperature stress,D. officinale ‘Jing Pin No.1’ seedlings were sprayed with different dilution multiples seaweed fertilizer(500,1000,1500)on the leaves and treated with a low-temperature of 4℃. The results showed that under low-temperature stress,the 1000 dilution multiples of seaweed fertilizer treatment alleviated the damage of leaf cell and chlorophyll degradation,decreased the malondialdehyde(MDA)content,while they also induced the proline content,peroxidase(POD),superoxide dismutase(SOD),ascorbate peroxidase(APX)activity and POD,SOD gene expression level,but seaweed fertilizer treatments reduced the catalase(CAT)activity and CAT gene expression level. In the early stages of the recovery growth period after low-temperature stress,the seaweed fertilizer treatments significantly increased the activity of antioxidant enzymes than control,which improved the comprehensive antioxidative capacity of D. officinale seedlings during the recovery growth period. In summary,spraying a suitable concentration seaweed fertilizer could improve the ability of resisting low-temperature for D. officinale.
Keywords:seaweed fertilizer  Dendrobium officinale’Jing Pin No  1’  low-temperature stress  antioxidant physiology  gene expression
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