| 应用ISSR-PCR对10个菊花品种进行遗传多样性分析 |
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| 引用本文： | 程华,李琳玲,张心玲,方佳,郑永生,姜德志,程水源.应用ISSR-PCR对10个菊花品种进行遗传多样性分析[J].湖北农业科学,2011(20):4292-4297. |
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| 作者姓名： | 程华 李琳玲 张心玲 方佳 郑永生 姜德志 程水源 |
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| 作者单位： | 经济林木种质改良与资源综合利用湖北省重点实验室;黄冈师范学院化学与生命科学学院;麻城市福白菊产业协会; |
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| 基金项目： | 湖北省自然科学基金项目(2010CDZ026); 湖北省教育厅优秀中青年科技创新团队项目(T200702) |
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| 摘 要： | 中国的菊花(Chrysanthemum morifolium Ramat.)品种数量众多,形态变异丰富,适应性强,分布广泛,遗传背景非常复杂,这为菊花品种的资源调查、分类鉴定、遗传多样性分析等带来了困难。试验采用分子标记技术,对福白菊(C.morifolium cv.Fubaiju)、杭白菊(C.morifolium cv.Hangbaiju)等10个菊花品种进行了分类鉴定及亲缘关系研究,结果显示,在简单序列重复区间扩增-聚合酶链式反应(Inter-simple sequence repeat-Polymerase chain reaction,ISSR-PCR)分子标记反应体系及引物的筛选方面,选择了条带数量多和清晰度高的菊花反应体系,应用野生福白菊对39条ISSR引物进行了筛选,淘汰了扩增效果差、带型不易辨认的引物,最终确定12条引物用于菊花品种的鉴定。ISSR-PCR聚类分析结果表明,12条ISSR引物扩增出了185条清晰的、重复性好的ISSR条带,其中多态性条带有176条,多态性比率高达95.1%。应用NTSYS 2.10e软件进行UPGMA法聚类分析,在相似系数0.55处可将10个菊花品种大致分为3类,结果福白菊与其他杭菊品种在遗传上有较大的差异。
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| 关 键 词： | 菊花 简单序列重复区间扩增-聚合酶链式反应 分子标记 遗传多样性 |
Genetic Diversity Analysis of Ten Chrysanthemum morifolium Cultivars Using ISSR-PCR |
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| CHENG Hua,LI Lin-ling,ZHANG Xin-ling,FANG Jia,ZHENG Yong-sheng,JIANG De-zhi,CHENG Shui-yuan.Genetic Diversity Analysis of Ten Chrysanthemum morifolium Cultivars Using ISSR-PCR[J].Hubei Agricultural Sciences,2011(20):4292-4297. |
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| Authors: | CHENG Hua LI Lin-ling ZHANG Xin-ling FANG Jia ZHENG Yong-sheng JIANG De-zhi CHENG Shui-yuan |
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| Institution: | CHENG Hua1,2,LI Lin-ling1,ZHANG Xin-ling3,FANG Jia2,ZHENG Yong-sheng3,JIANG De-zhi1,CHENG Shui-yuan1,2(1.Economic Forest Germplasm Improvement and Comprehensive Utilization of Resources of Hubei Key Laboratories,Huanggang 438000,Hubei,China,2.College of Chemistry and Life Science,Huanggang Normal University,3.The Fubaiju Industry Association of Macheng City,Macheng 438331,China) |
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| Abstract: | The investigation,classification,identification and diversity analysis of the Chrysanthemum morifolium Ramat.were difficulty due ta its numerous cultivars,abundant morphological variation,powerful suitability,wide distribution and extremely complicated genetic background.The classification and genetic relationship of 10 C.morifolium cultivars were studied using cluster analysis of molecular markers.The main results were as follows.The chrysanthemum reaction system with more bands and high-definition was sel... |
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| Keywords: | Chrysanthemum morifolium Ramat Inter-simple sequence repeat-Polymerase chain reaction(ISSR-PCR) molecular marcer genetic diversity |
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